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园艺学报 ›› 2012, Vol. 39 ›› Issue (11): 2265-2270.

• 研究报告 • 上一篇    下一篇

离体雌核发育诱导洋葱单倍体与植株再生

 刘冰江, 缪军, 霍雨猛, 杨妍妍, 徐坤, 吴雄   

  1. (1 山东农业大学园艺科学与工程学院,山东泰安 271018;2 山东省农业科学院蔬菜研究所,山东省设施蔬菜生物学重点实验室,国家蔬菜改良中心山东分中心,济南 250100)
  • 出版日期:2012-11-25 发布日期:2012-11-25

Haploid Plant Induction via in Vitro Gynogenesis and Plant Regeneration in Onion(Allium cepa L.)

LIU  Bing-Jiang, MIAO  Jun, HUO  Yu-Meng, YANG  Yan-Yan, XU  Kun, WU  Xiong   

  1. (1College of Horticultural Sciences and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China;2Vegetable Research Institute of Shandong Academy of Agricultural Sciences,Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province,National Improvement Center for Vegetable,Shandong Branch,Ji’nan 250100,China)
  • Online:2012-11-25 Published:2012-11-25

摘要: 以来自日本的3个中日照型洋葱(Allium cepa L.)杂交种的未开放花蕾为外植体材料,研究了2,4-D和6-BA浓度及配比对单倍体诱导培养的影响,以建立洋葱单倍体诱导技术体系。研究结果表明,含有1.5 mg · L-1 2,4-D + 1.5 mg · L-1 6-BA和2.0 mg · L-1 2,4-D + 2.0 mg · L-1 6-BA的B5培养基适于洋葱单倍体的诱导,诱导率最高达到4.00%。共获得了43枚雌核发育胚,雌核发育胚转移至不含激素的B5基本培养基中即可发育成完整植株,再生植株驯化移栽成活率达到100%。利用根尖染色体计数和流式细胞仪鉴定有42株是单倍体。

关键词: 洋葱, 雌核发育, 单倍体, 流式细胞仪

Abstract: The flower buds of three intermediate-day hybrid cultivars of onion(Allium cepa L.)from Japanese seed company were cultured in vitro using B5 medium which supplied with 100 g · L-1 sucrose and 2,4-D and 6-BA. The suitable media for haploid induction of onion were B5 medium with 1.5 mg · L-1 2,4-D + 1.5 mg · L-1 6-BA and B5 medium with 2.0 mg · L-1 2,4-D + 2.0 mg · L-1 6-BA. The highest induction rate was 4.00%. Forty-three gynogenic embryos were obtained and each of them developed into the whole plant on the B5 medium with no any plant growth regulators. The regenerated plantlets were domesticated and transplanted in greenhouse. The survival rate was 100%. Ploidy analysis of the regenerants with chromosome counts of root tip cells and flow cytometry analysis of leaf tissue revealed that 42 of them are haploid.

Key words: onion, Allium cepa L., gynogenesis, haploid, flow cytometry

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