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园艺学报 ›› 2012, Vol. 39 ›› Issue (11): 2182-2188.

• 蔬菜 • 上一篇    下一篇

芦笋超雄株的DNA 分子标记辅助筛选

 周劲松, 汤泳萍, 罗绍春, 谢启鑫, 张岳平, 陈光宇   

  1. (江西省农业科学院蔬菜花卉研究所,江西省农业科学院生物工程中心,南昌 330200)
  • 出版日期:2012-11-25 发布日期:2012-11-25

Selection of Super-male Plants in Asparagus with DNA Molecular Marker

 ZHOU  Jin-Song, TANG  Yong-Ping, LUO  Shao-Chun, XIE  Qi-Xin, ZHANG  Yue-Ping, CHEN  Guang-Yu   

  1. (Institute of Vegetables and Flowers/Biotechnology Center,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China)
  • Online:2012-11-25 Published:2012-11-25

摘要: 以芦笋两性株自交S1代雄株为试验材料,首先与优良雌株进行测交,然后利用与芦笋性别决定基因紧密连锁的显性DNA分子标记对苗期各单株进行性别鉴定,推断出超雄株,为芦笋全雄育种提供亲本材料。研究结果表明:显性标记Asp1-T7和Asp1-T7sp特异性好,扩增效果稳定,均能够鉴别芦笋雌、雄性别;一共测验了14株芦笋两性株自交S1代的雄株,从中筛选出2株超雄株;对田间测交组合单株显花后性别表型调查的结果表明,DNA分子标记检测结果与田间实际相符,2株超雄株所对应的组合被确证为全雄组合。

关键词: 芦笋, 超雄株, DNA 分子标记辅助选择, 测交

Abstract: DNA molecular marker assisted selection of super-male plants was conducted for all-male breeding of asparagus.Males in S1 population of andromonoecious plants were test-crossed with elite females. Two DNA molecular markers tightly linked to sex determining gene M,Asp1-T7 and Asp1-T7sp,which showed stable and specific amplification,were used for sex identification of individuals in the progeny at seedling stage.Putative‘supermales’(MM)were differentiated from the normal males(Mm) by the presence or absence of‘all-male’population in the progeny. Two out of 14 male plants test crossed were distinguished as putative‘supermales’. The test result of DNA molecular marker-assisted selection was highly consistent with that of the sexual phenotype in field survey.The corresponding combinations originated from the two super-male plants were also identified as all-male combinations.

Key words: Asparagus officinalis, super-male plant, DNA molecular Marker-Assisted Selection, testcross

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