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园艺学报 ›› 2012, Vol. 39 ›› Issue (10): 2015-2022.

• 研究报告 • 上一篇    下一篇

番茄脱水素基因SlDHN2b 的克隆与表达分析

郭鹏,张士刚,金华,邹吉祥,董燕,姜国斌   

  1. (1 大连民族学院环境与资源学院生物技术系,辽宁大连 116600;2 山东泰安市农业局,山东泰安 271018;3 辽宁林业职业技术学院,沈阳 110101)
  • 出版日期:2012-10-25 发布日期:2012-10-25

Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato

GUO  Peng, ZHANG  Shi-Gang, JIN  Hua, ZOU  Ji-Xiang, DONG  Yan, JIANG  Guo-Bin   

  1. (1College of Environment and Resources,Dalian Nationalities University,Dalian,Liaoning 116600,China;2Tai’an Municipal Bureau of Agriculture,Tai’an,Shandong 271018,China;3Liaoning Forestry Vocational and Technical College,Shenyang 110101,China)
  • Online:2012-10-25 Published:2012-10-25

摘要: 利用RT-PCR从番茄叶片中获得脱水素基因(dehydrins)的cDNA序列,命名为SlDHN2b。该基因开放阅读框1 140 bp,编码380个氨基酸。蛋白序列分析表明该蛋白高度亲水。Real-time PCR分析表明该基因受盐、脱水、ABA诱导表达。通过染色体步移技术获得SlDHN2b基因的启动子,PLACE数据库分析预测SlDHN2b启动子序列中含有多种逆境相关的作用元件。Northern杂交表明该基因在叶片中表达量最高,果实、花、茎中次之,根中最少。

关键词: 番茄, SlDHN2b, 克隆, 逆境, 启动子

Abstract: In present study,a cDNA clone,designated SlDHN2b,was isolated from tomato by RT-PCR. The ORF of SlDHN2b was 1 140 bp and contained 380 amino acid residues. Protein sequence analysis showed that the SlDHN2b was high hydrophilic. Real-time PCR analysis indicated that mRNA accumulation of SlDHN2b was induced by salt stress,abscisic acid(ABA)and dehydration. Analysis of the promoter of PdEPF1 revealed the presence of stress-responsive elements by genome walking kit. Tissue-specific expression indicated that SlDHN2b was mainly expressed in leaves,weakly expressed in bud,flower,stem and the lowest in roots. Analysis of the promoter of SlDHN2b revealed the presence of stress-responsive elements.

Key words: tomato, SlDHN2b, cloning, abiotic stress, promoter

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