关键词: 月季, CBF 转录因子, 基因, 克隆, 表达

Abstract: We firstly cloned the partial fragment of CBF from Rosa hybrida‘Hanjin 4’using homology cloning methods according to AP2/EREBP conserved sequences. Then，two pairs of specific primers for inverse PCR were designed to clone the 5' and 3' end sequences of CBF gene，respectively. The resulting 5' and 3' end sequences and the partial fragment were combined to a full length sequence，which was named RhCBF and submitted to GenBank（Accession number：EF582843）. The full-length of cDNA is 758 bp and the open reading frame（ORF）is 603 bp，encoding 200 amino acids；According to Real-time PCR analysis using a pair of specific primers，we found that salt，low temperature stresses could induce the expression of RhcCBF dramatically，but the mRNA level of RhCBF could hardly induced by drought treatment. These results show that RhCBF would play a critical role in salt and low temperature stresses.

Key words: rose, transcription factor CBF gene, gene, cloning, expression