关键词: 大白菜, 拟南芥, BpNFYA5, 基因, 抗旱性, 功能

Abstract: Through alignment with the sequence of NFYA5 gene from Arabidopsis thaliana（At1g54160），we got the homologous ESTs in Chinese cabbage[Brassica campestris L. ssp. pekinensis（Lour.）Makino]. Then，the full length of the gene namely BpNFYA5 was cloned by the method of Electronic Clone. The deduced protein of BpNFYA5 has the CCAAT-box binding domain，which showed 92.6% amino acid sequence identify with that of Arabidopsis. BpNFYA5 transcript is strongly induced by drought stress. The over-expression vector pBIn-NFYA5 and RNA-interference vector pFGC-NFYA5 were constructed and transformed to Arabidopsis thaliana（Col）mediated by Agrobacterium tumefaciens. The drought-tolerance was analyzed between the BpNFYA5 over-expression（OL），RNA-interference（Ri） and the wild-type（WT）Arabidopsis through the methods of drought stress and 10% PEG6000 drought simulated treatments. The results showed that chlorophyll content of OL was higher compared with those of WT and Ri；Proline content of OL accumulated quickly and showed higher than those of WT and Ri； Likewise，the rate of water loss in OL leaves was less than in WT and Ri. All above suggested that BpNFYA5 gene play an important role in drought response.

Key words: Chinese cabbage, Arabidopsis, BpNFYA5, gene, drought-tolerance