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园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1681-1684.

• 研究简报 • 上一篇    下一篇

短期高温胁迫对一品红幼苗抗氧化系统的影响

陶 俊1*,俞 菊1,俞丽琴1,苏家乐2,张才红2   

  1. (1扬州大学园艺与植物保护学院,江苏扬州 225009;2江苏省农业科学院园艺研究所,南京 210014)
  • 收稿日期:2008-05-22 修回日期:2008-09-18 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 陶 俊

Effects of Short-term High Temperature Stress on Antioxidant System in Poinsettia

TAO Jun1*;YU Ju1;YU Li-qing1;SU Jia-le2;and ZHANG Cai-hong2
  

  1. (1College of Horticulture and Plant Protection, Yangzhou University, Yangzhou,Jiangsu 225009, China; 2Institute of Horticulture, Jiangsu Academy of Agricultural sciences, Nanjing 210014, China)
  • Received:2008-05-22 Revised:2008-09-18 Online:2008-11-25 Published:2008-11-25
  • Contact: TAO Jun

摘要:

以一品红'天鹅绒'幼苗为试材,利用人工气候箱研究了昼45 ℃/夜38 ℃的高温胁迫处理对植株叶片相对电导率、产生速率 、H2O2及丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性的影响。结果表明,在高温胁迫下,CAT、APX、SOD酶活性先升高,然后各自在0.5 d、1 d、2 d时分别转为下降趋势;而POD酶活性则一直增加;相应地,2 d内一品红的膜透性变化不大,2 d后急剧增大;MDA含量2 d内先升后降,2 d后大幅度上升;产生速率 、H2O2含量在2 d后均快速上升。2 d是'天鹅绒'一品红耐受昼45 ℃/夜38 ℃高温胁迫的极限时间。

关键词: 高温胁迫, 一品红, 膜透性, 丙二醛, 活性氧, 保护酶

Abstract: The relative electrical conductivity, production rate, content of H2O2 and malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) activity were measured in potted poinsettia (Red Velvet) after treatment with high temperature in 45 ℃ /38 ℃ (day/ night) in artificial climatic chambers. The results showed that SOD, CAT and APX activity increased at first, and then decreased in 0.5 d, 1 d, 2 d, respectively, while POD activity increased all the time. Correspondingly, the membrane permeability increased a little within two days and then rose suddenly. The content of MDA firstly increased, then slightly deceased within two days, and then had a large scale rise after two days. At the same time, production rate and H2O2 content enhanced rapidly after two days.Therefore, two days maybe the time limit of tolerance of potted poinsettia (Red Velvet) to high temperature of 45 ℃ /38 ℃ (day/ night).

Key words: high temperature stress, poinsettia, membrane permeability, malondialdedyle, reactive oxygen species, defensive enzyme

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