茄子果形的QTL 定位

园艺学报 ›› 2012, Vol. 39 ›› Issue (6): 1115-1122.

茄子果形的QTL 定位

乔军，陈钰辉，王利英，刘富中，张映，连勇

（1 中国农业科学院蔬菜花卉研究所，北京 100081；2 天津科润蔬菜研究所，天津 300384）

出版日期:2012-06-25 发布日期:2012-06-25

QTL Analysis for Fruit Shape in Eggplant Based on Genetic Linkage Map

QIAO Jun, CHEN Yu-Hui, WANG Li-Ying, LIU Fu-Zhong, ZHANG Ying, LIAN Yong

（1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081；2Tianjin Kernel Vegetable Research Institute，Tianjin 300384，China）

Online:2012-06-25 Published:2012-06-25

摘要/Abstract

摘要： 以圆茄高代自交系106和长茄高代自交系113为亲本，利用其F₂群体构建了一张包括23个SSR标记和85个AFLP标记，共15个连锁群的复合遗传图谱。该图谱覆盖基因组长度1 007.9 cM，平均图距9.3 cM，长度和密度符合定位标准。采用MapQTL4.0软件并结合MQM作图法对果形QTL进行定位分析，定位到与果形指数相关的两个QTL，位于第1和第12连锁群上，表型贡献率分别为20.8%和41.5%；与果长相关的5个QTL，位于第1、8、11、12、14连锁群上，表型贡献率分别为16.5%、36.8%、9.8%、45.0%和41.9%；与果径相关的两个QTL，位于第1和第5连锁群上，表型贡献率分别为16.2%和15.8%。果形指数、果长和果径QTL同时定位在第1连锁群上，且与AFLP标记M23E21B距离3.5 cM，表明果形性状与该标记紧密连锁。

关键词: 茄子, 果形, 分子遗传图谱, QTL

Abstract: An AFLP and SSR genetic linkage map was constructed in eggplant（Solanum melongena L.）with an intraspecific F₂ population derived from a cross between the round eggplant inbred line‘106’and the long eggplant inbred line‘113’. The generated map consisted of 15 linkage groups with 85 AFLP markers and 23 SSR markers，a total coverage of 1 007.9 cM and an average interval of 9.3 cM. The markers distributed randomly across the groups and could be done preliminary QTL mapping in MQM using MapQTL4.0. Two QTLs for fruit shape index were in the 1st and 12th linkage group, and explained 20.8% and 41.5% of phenotypic variances；Five QTLs for fruit length were in the 1st，8th，11th，12th and 14th linkage group，and explained 16.5%，36.8%，9.8%，45.0% and 41.9% phenotypic variances；And two QTLs for fruit width were detected in the 1st and 5th linkage group explaining 16.2% and 15.8% of phenotypic variances. QTLs for fruit shape index，fruit length and fruit diameter were located in the 1st linkage group，and their distance was 3.5 cM with the AFLP marker M23E21B which was closely linked to the fruit shape traits.

Key words: eggplant, fruit shape, genetic linkage map, QTL

中图分类号:

S 641.1

乔军, 陈钰辉, 王利英, 刘富中, 张映, 连勇. 茄子果形的QTL 定位[J]. 园艺学报, 2012, 39(6): 1115-1122.

QIAO Jun, CHEN Yu-Hui, WANG Li-Ying, LIU Fu-Zhong, ZHANG Ying, LIAN Yong. QTL Analysis for Fruit Shape in Eggplant Based on Genetic Linkage Map[J]. ACTA HORTICULTURAE SINICA, 2012, 39(6): 1115-1122.

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