关键词: 结球甘蓝, VIN3, 春化作用, 基因表达

Abstract: The mid harvest season cabbage variety‘Guangrong 1’was used in this experiment. Fourteen leaves cabbage plants were vernalized in the incubator at 8 ℃. Two vernalization-related geneswere isolated by RT-PCR. Sequencing analysis results showed that their ORF were both 1 680 bp in length，which encoded 560 amino acids，and GenBank accession number are JQ394927 and JQ394928 respectively. Their nucleotide sequences shared 85.55% and 79.95% identity with AtVIN3 respectively，and the deduced amino acid sequence showed homology to AtVIN3 with 80.72% and 72.96%. The expression patterns of the BoVIN3 genes were investigated by semi-quantity RT-PCR，results showed that BoVIN3 gene could express induced by low temperature treatment，and expressed in the stem apex specifically，transcript levels increased with increasing treatment time. The transcription reach the peak at 42 d of vernalization. At the same time the expression of FLC was suppressed after BoVIN3 wasinduced by low temperature treatment，results suggest that both genes maybe involved in the biological process of flowering regulation in vernalization. However both BoVIN3 could not be induced by GA₃ and KT treatment. It indicated that cabbage BoVIN3was specific vernalization genes，whose transcripts could specially response to vernalization.

Key words: cabbage, VIN3, vernalization, gene expression