https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1587-1594.

• 果树 • 上一篇    下一篇

反义乙烯受体FaEtr2基因对草莓的转化

朱海生1,2,3,潘东明1*,温庆放2,3*,林义章1
  

  1. (1福建农林大学园艺学院,福州 350002;2福建省农业科学院蔬菜研究中心,福州 350013;3福建省农业科学院作物研究所,福州 350013)
  • 收稿日期:2008-06-24 修回日期:2008-10-14 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 潘东明,温庆放

Transformation of Antisense Ethylene Receptor FaEtr2 Gene in Strawberry

ZHU Hai-sheng1,2,3;Dong-ming1*;WEN Qing-fang2,3*;and LIN Yi-zhang1   

  1. (1College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2Vegetable Research Center, Fujian Academy of Agricultural Sciences Fuzhou 350013, China;3Crops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China)
  • Received:2008-06-24 Revised:2008-10-14 Online:2008-11-25 Published:2008-11-25
  • Contact: PAN Dong-ming,WEN Qing-fang

摘要:

通过根癌农杆菌EHA105介导转化草莓叶片,在卡那霉素选择压下连续分化选择、扩繁和生根培养,获得了26株转乙烯受体FaEtr2反义基因植株。PCR和Southern blot检测证明,该基因已经整合草莓染色体中。Northern分析表明,转入的反义基因对靶基因都有部分的抑制。对果实乙烯释放量测定表明,转基因草莓中乙烯释放量降低。

关键词: 草莓, 乙烯受体, 转化

Abstract:

Improving keeping quality of strawberry fruit is a difficult problem. Applying antisense gene strategy to control ethylene receptor is a new feasible method. The antigene FaEtr2 was introduced into leaves of stawberry(Fragaria ananassa Duch.)with Agrobacterium tumefaciens,and 26 transgenic plants were selected under kanamycin. It was proved that anti- FaEtr2 was integrated with chromosome of transgenic plant,according to the results of PCR and Southern blot. Northern blot analysis indicated that FaEtr2 mRNA abundance was decresased in antisense strawberry plants. The ethylene production in transgenic strawberry significantly decreased. The results suggest that there is some intricate relation between FaEtr2 and the ripening of strawberry fruits. The anti-transgenic strawberry provide precious materials for the further research.

Key words: strawberry, ethylene receptors, genetic transformation

中图分类号: