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园艺学报 ›› 2012, Vol. 39 ›› Issue (5): 845-852.

• 果树 • 上一篇    下一篇

‘泰山早霞’苹果采后1–甲基环丙烯处理对其软化及相关基因表达的影响

刘美艳, 魏景利, 刘金, 房龙, 宋杨, 崔美, 王传增, 陈学森   

  1. (1 山东农业大学作物生物学国家重点实验室,山东泰安 271018;2 山东省冠县国有毛白杨林场,山东冠县 252500)
  • 出版日期:2012-05-25 发布日期:2012-05-25

The Regulation of 1-methylcyclopropene on Softening and Expression of Relevant Genes in‘Taishan Zaoxia’Apple

LIU  Mei-Yan, WEI  Jing-Li, LIU  Jin, FANG  Long, SONG  Yang, CUI  Mei, WANG  Chuan-Zeng, CHEN  Xue-Sen   

  1. (1State Key Laboratory of Crop Biology,Shandong Agricultural Uiversity,Tai’an,Shandong 271018,China;2Guanxian State Forest Farm of Populas Tomentosa,Guanxian,Shandong 252500,China)
  • Online:2012-05-25 Published:2012-05-25

摘要: 以‘泰山早霞’苹果成熟的果实为试材,于采收当天进行1–甲基环丙烯(1-MCP)处理,研究其对果实软化及相关基因表达的影响。结果表明,①1-MCP处理的果实在短期贮藏期间硬度均明显高于对照。②1-MCP处理后,乙烯释放速率、PGPMEβ-Galα-L-AfLOX等细胞壁酶基因的表达均被明显抑制,在整个试验期内均有明显下降,尤其是在处理后1 d,就分别比各自的对照下降了72.0%、72.1%、87.5%、81.8%、90.2%和16.7%,而1-MCP对AMXET基因的表达没有明显作用。上述结果表明,苹果‘泰山早霞’品种属乙烯极敏感型,1-MCP对于延缓其果实软化具有明显作用,其果实软化可能是PGPMEβ-Galα-L-AfLOX等多种基因协同作用的结果。

关键词: 苹果, 早熟, 贮藏, 软化, 1-MCP, 细胞壁酶, 基因, 表达

Abstract: In order to study the molecular softening mechanism of‘Taishan Zaoxia’apple,the effect of 1-MCP on softening and expressions of genes related to softening in‘Taishan Zaoxia’were investigated. The results showed that:①The firmness of‘Taishan Zaoxia’fruit treated with 1-MCP were higher than the control fruit during the storage after harvest.②Ethylene production rate and the expression of PGPMEβ-Galα-L-Af and LOX genes of‘Taishan Zaoxia’fruit were inhibited significantly throughout the whole experimental period after treated with 1-MCP,especially in the first day after treatment. They decreased 72.0%,72.1%,87.5%,81.8%,90.2% and 16.7% than control respectively. But 1-MCP had no effect on expression of AM and XET genes. These results indicated that‘Taishan Zaoxia’apple variety was very sensitive to ethylene and 1-MCP could delay softening of‘Taishan Zaoxia’fruit obviously;The fruit  softening may be caused by synergistic effect of PGPMEβ-Galα-L-Af and LOX genes.

Key words: apple, early-maturing, storage, softening, 1-MCP, cell wall enzyme, gene, express