大花惠兰根腐病拮抗细菌ZL7-5菌株的筛选与鉴定

园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1647-1652.

大花惠兰根腐病拮抗细菌ZL7-5菌株的筛选与鉴定

李潞滨¹,李术娜²,李佳²,王倩²,朱宝成^2*,彭镇华^1*

（¹中国林业科学研究院林业研究所，国家林业局林木培育重点实验室，北京 100091；²河北农业大学,生命科学学院，河北保定 071001）

收稿日期:2008-05-12 修回日期:2008-07-21 出版日期:2008-11-25 发布日期:2008-11-25
通讯作者: 朱宝成,彭镇华

Isolation and Identification of the Antagonistic Bacterial Strain ZL7-5 Against Root Rot Disease of Cymbidium hybridium

LI Lu-bin¹;LI Shu-na²;LI Jia²;WANG Qian²;ZHU Bao-cheng^2*;and PENG Zhen-hua^2*

(¹Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China; ²College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China)

Received:2008-05-12 Revised:2008-07-21 Online:2008-11-25 Published:2008-11-25
Contact: ZHU Bao-cheng and PENG Zhen-hua

摘要/Abstract

摘要：

从各地大花惠兰种植地采集的土样中共分离到细菌519株，初筛出对大花惠兰根腐病病原菌尖孢镰刀菌（Fusarium oxysporium）具有拮抗活性的细菌菌株26株，经过复筛，选出1株具有较高拮抗活性的菌株ZL7-5。通过16S rDNA序列相似性分析，菌株ZL7-5与解淀粉芽孢杆菌标准菌株(NBRC 15535)的16S rDNA序列相似度达100%，通过比较菌株ZL7-5和模式菌株的形态特征和生理生化特性，最终将菌株ZL7-5鉴定为解淀粉芽孢杆菌（Bacillus amyloliquefaciens）。

关键词: 大花惠兰, 根腐病, 尖孢镰刀菌, 拮抗细菌, 筛选, 鉴定, 解淀粉芽孢杆菌

Abstract:

In order to obtain antagonistic strain which has the potential application possibility on biocontrol of root rot disease of Cymbidium hybridium, five hundreds and nineteen bacterial strains were isolated from the soil samples and there were twenty six bacterial strains showed antagonistic activity to Fusarium oxysporium.A strainwith the highest antagonistic activity, ZL7-5, was obtained during the secondary screening. 16S rDNA sequences analysis of strain ZL 7-5 revealed that it had 100% similarity to that of Bacillus amyloliquefaciens NBRC 15535. After the morphological, physiological and biochemical characteristics of this strain were further studied and compared with that of the type strain Bacillus amyloliquefaciens NBRC 15535, strain ZL7-5 was finally identified as Bacillus amyloliquefaciens.

Key words: Cymbidium hybridium, root rot, Fusarium oxysporium, antagonistic bacteria, screening, identification, Bacillus amyloliquefaciens

中图分类号:

S 682.31

李潞滨;李术娜;李佳;王倩;朱宝成;彭镇华. 大花惠兰根腐病拮抗细菌ZL7-5菌株的筛选与鉴定[J]. 园艺学报, 2008, 35(11): 1647-1652.

LI Lu-bin;LI Shu-na;LI Jia;WANG Qian;ZHU Bao-cheng;and PENG Zhen-hua. Isolation and Identification of the Antagonistic Bacterial Strain ZL7-5 Against Root Rot Disease of Cymbidium hybridium[J]. ACTA HORTICULTURAE SINICA, 2008, 35(11): 1647-1652.

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