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园艺学报 ›› 2012, Vol. 39 ›› Issue (4): 621-628.

• 果树 • 上一篇    下一篇

DA-6 对桃树叶片叶绿素合成途径的调控研究

 杨清, 艾沙江 · 买买提, 王志霞, 刘国杰   

  1. (中国农业大学农学与生物技术学院果树系,北京 100193)
  • 出版日期:2012-04-25 发布日期:2012-04-25

Effects of DA-6 on Chlorophyll Biosythesis Pathway in Peach Leaves

 YANG  Qing, AI  Sha-Jiang- · Mai-Mai-Ti, WANG  Zhi-Xia, LIU  Guo-Jie   

  1. (Department of Pomology,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China)
  • Online:2012-04-25 Published:2012-04-25

摘要: 以‘香山水蜜桃’为试材,研究叶面喷施DA-6(二烷氨基乙醇羧酸酯N,N-diethylaminoethyl hexanote)对叶绿素(Chlorophyll,Chl.)合成过程中前体物质的含量以及合成关键酶活性的影响,探讨 DA-6 提高桃叶片Chl.含量的可能原因。结果表明:生长季叶面喷施20 mg · L-1 DA-6 显著提高了Chl.的含量,Chl.b 含量的提高尤为显著,降低了Chl.的前体物质5–氨基酮戊酸(5-aminolevulinic acid,ALA)含量,增加了后续中间产物胆色素原(porphobilinogen,PBG)、尿卟啉原Ⅲ(uroporphyrinoge Ⅲ,Urogen Ⅲ)含量,表明DA-6 处理促进了ALA 向PBG 的转化和PBG 向Urogen Ⅲ的转化。以上两步骤的关键酶5–氨基酮戊酸脱水酶(5-aminolevulinate dehydratase,ALAD)和尿卟啉原Ⅲ合酶(uroporporphyrinogn Ⅲ synthase,UROS)的活性在处理后显著提高。叶面喷施DA-6 是通过提高ALA 向PBG 和PBG 向Urogen Ⅲ的转化效率,促进Chl.的合成,使Chl.含量增加。

关键词: 桃, DA-6, 叶绿素, 合成, 前提物质, 关键酶, 酶活性

Abstract: Taken ‘Xiangshan Honey’ peach as the material,the change of chlorophyll precursor contents and activity of the key enzymes in chlorophyll biosynthesis pathway after DA-6 (N-diethylaminoethyl hexanote)application were investigated the underlying mechanism of DA-6 induced leaf chlorophyll content increase. Compared with the control,20 mg · L-1 foliar DA-6 spray significantly increased the contents of chlorophylls,especially that of chlorophyll b,and chlorophyll intermediate products porphobilinogen(PBG)and uroporphyrinoge(Urogen Ⅲ),whereas decreased the amount of chlorophyll precursor 5-aminolevulinic acid(ALA). The results suggested the transformation from ALA to PBG and PBG to Urogen Ⅲ was up-regulated. The key enzymes catalyzing the two reactions are 5-aminolevulinate dehydratase(ALAD)and uroporporphyrinogn Ⅲ synthase(UROS),their enhanced activity after DA-6 foliar spraying was assayed. Our study demonstrated that foliar spray DA-6 increased the conversion efficiency of ALA to PBG and PBG to Urogen Ⅲ,up-regulated the chlorophyll biosynthesis pathway and resulted in higher chlorophyll content in peach leaves.

Key words: peach, DA-6, chlorophyll, biosynthesis, precursors, key enzyme, enzyme activity

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