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园艺学报 ›› 2012, Vol. 39 ›› Issue (3): 436-442.

• 果树 • 上一篇    下一篇

香蕉A基因组6个品种的核型分析

劳世辉,盛  鸥,魏岳荣*,易干军,邓夫平   

  1. 农业部南亚热带果树生物学与遗传资源利用重点实验室,广东省农业科学院果树研究所,广州510640
  • 收稿日期:2011-12-18 修回日期:2012-02-08 出版日期:2012-03-25 发布日期:2012-03-25
  • 通讯作者: 魏岳荣 E-mail:weid18@163.com

Karyotype Analysis of Six Banana Cultivars in Relation to A Genomes

LAO Shi-hui,SHENG Ou,WEI Yue-rong*,YI Gan-jun,and DENG Fu-ping   

  1. Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization,Ministry of Agriculture of China;Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China
  • Received:2011-12-18 Revised:2012-02-08 Online:2012-03-25 Published:2012-03-25
  • Contact: WEI Yue-rong E-mail:weid18@163.com

摘要: 以6个A基因组香蕉(Musa spp.)品种为材料,采用改良的去壁低渗法对其核型特征进行分析。‘天宝蕉’(AAA)核型公式为2n = 3x = 33 = 3M + 27m(3SAT)+ 3sm,属“1A”核型;‘海南贡蕉’(AA)核型公式为2n = 2x = 22 = 2M + 18m(2SAT)+ 2sm,属“2A”核型;‘玫瑰蕉’(AA)、‘贡蕉’(AA)、‘抗枯1号’(AAA)和‘抗枯5号’(AAA)核型公式分别为2n = 2x = 22 = 2M + 16m(SAT)+ 2sm + 2st,2n = 2x = 22 = 4M + 16m(SAT)+ 2sm,2n = 3x = 33 = 6M + 15m(3SAT)+ 12sm和2n = 3x = 33 = 6M + 15m(3SAT)+ 12sm(3SAT),属“2B”核型。基于核型分析结果,绘制了6个香蕉品种的核型模式图。

关键词: 香蕉, 染色体特征, 核型分析

Abstract: Karyotype analysis was performed to investigate the chromosome features of 6 banana (Musa spp.) cultivars in relation to A genomes by using the method of wall degradation hypotonic. Results showed that the analysed cultivars of diploid or triploid that contained only A genomes,viz. AA or AAA,varied in karyotype characteristics. The karyotype formula and its type were 2n = 3x = 33 = 3M + 27m(3SAT)+ 3sm and“1A”for‘Tianbaojiao’(AAA),2n = 2x = 22 = 2M + 18m(2SAT)+ 2sm and“2A”for’Hainan Gongjiao’(AA),respectively. The karyotype formula for‘Rose’(AA),‘Pisang Mas’(AA),‘Kangku 1’(AAA)and‘Kangku 5’(AAA)were 2n = 2x = 22 = 2M + 16m(SAT)+ 2sm + 2st,2n = 2 x = 22 = 4M + 16m(SAT)+ 2sm,2n = 3x = 33 = 6M + 15m(3SAT)+ 12sm,2n = 3 x = 33 = 6M + 15m(3SAT)+12sm(3SAT),respectively,whereas all these four cultivars exhibited a karyotype asymmetry of type“2B”. The idiogram was established for the analysed banana cultivars.

Key words: Musa, chromosome features, karyotype analysis