关键词: 葡萄, 重金属, 钙, 镉, 保护酶, 呼吸作用

Abstract:

Protecting effects of CaCl₂ on the leaves of grape against cadmium stress were studied by root treatment using cutting seedling of Kyoho grape. The results showed that CdCl₂ could induce significantly accumulation of MDA and H₂O₂ and increase electrolytic leakage of PM (plasma membrane), also markedly inhibited the respiratory rate, CAT activity, H⁺-ATPase and Ca²⁺-ATPase activities in PM. The application of 0.5 mmol·L^-1 CaCl₂ inhibited significantly the increases of MDA and H₂O₂ content and electrolytic leakage of PM caused by CdCl₂ treatment, and decreased the inhibition of CAT activity, respiratory rate and the activity of H⁺-ATPase and Ca²⁺-ATPase in PM under CdCl₂ treatment. These indicated that 0.5 mmol·L^-1 CaCl₂ can alleviate the stress caused by CdCl2 in grape leaves.

Key words: grape, heavy metal, calcium, cadmium, antioxidant enzymes, respiration