https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2011, Vol. 38 ›› Issue (8): 1531-1538.

• 研究报告 • 上一篇    下一篇

贵州樱桃种质资源的ISSR分析

宋常美1,2,文晓鹏1,*,杨尔泰3   

  1. 1贵州大学,贵州省农业生物工程重点实验室,贵阳550025;2贵阳学院,贵阳550025;3贵州大学生命科学学院,贵阳550025
  • 收稿日期:2011-04-27 修回日期:2011-08-01 出版日期:2011-08-25 发布日期:2011-08-25
  • 通讯作者: 文晓鹏1,*

Cherry Germplasm from Guizhou Province Analyzed by ISSR Markers

SONG Chang-mei1,2,WEN Xiao-peng1,*,and YANG Er-tai3   

  1. 1Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang 550025,China;2Guiyang College,Guiyang 550025,China;3College of Life Sciences,Guizhou University,Guiyang 550025,China
  • Received:2011-04-27 Revised:2011-08-01 Online:2011-08-25 Published:2011-08-25
  • Contact: WEN Xiao-peng1,*

摘要: 以64份贵州地方樱桃(Prunus pseudocerasus L.)种质资源及栽培品种黑珍珠为试材进行ISSR分析。结果表明:用从90条ISSR引物中筛选出的21条稳定性强、条带清晰及多态性丰富的引物进行PCR扩增,共获得228个标记,其中多态性标记为199个,多态性比例为87.28%;采用NTSYS 2.01软件计算,相似系数为0.49 ~ 0.90,表明贵州地方樱桃资源的多样性丰富;UPGMA聚类分析显示,毕节、遵义及黔南等地资源具有丰富的多样性,尤其毕节地区拥有大量品质优良的资源,可作为品质育种的亲本。

关键词: 樱桃, 种质资源, ISSR, 遗传多样性

Abstract: Sixty-four cherry(Prunus pseudocerasus L.)accessions from Guizhou Province,as well as the cultivar Black Pearl,were analyzed by ISSR markers. A total of 228 markers were scored from the 21 primers,which were screened out from 90 primers and demonstrated highly reproducible,clear and polymorphic bands. Among the obtained markers,199 were polymorphic,accounting for 87.28% of the total. Analyzed by NTSYS2.01,the genetic similarity coefficient among the accessions ranged from 0.49 to 0.90,reflecting the high genetic diversity of cherry germplasms in Guizhou Province. The UPGMA dendrogram demonstrated that the accessions from Bijie,Zunyi and Qiannan areas were genetically more diversified. In particular the accessions from Bijie exhibited the highest degree of diversity in fruit quality,which may be used as elite materials for further genetic improvement in this species.

Key words: cherry, germplasm, ISSR, genetic diversity

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