关键词: 果树, 安全标记基因, 转基因, 甘露糖, 正向选择

Abstract: Despite the production of fertile transgenic plants through transformation mediated by Agrobacterium tumefaciens，transformation efficiency is still low in fruit trees. The mannose selection system employs the phosphomannose isomerase（PMI）gene as selectable gene and the transgenic PMI-expressing cells have acquired the ability to convert mannose-6-phosphate to fructose-6-phosphate. Compare to antibiotic selection system，it has advantages such as high selective efficiency，safety for the environment and easy to test. It is also affected by some important factors. Here we talk about the application of this system on fruit trees transformation and its perspective based on the existing literature data. The advantages of this select system，some important factors affecting the transformation efficiency are discussed as well as the approaches in fruit tree genetic transformation using this PMI/mannose system.

Key words: fruit tree, safe marker gene, transgenic, mannose, positive-selection