关键词: 银耳, α–微管蛋白, 克隆, 序列分析, 系统发育树

Abstract: Partial cDNA sequence of α-tubulin gene was amplified from Tremella fuciformis by degenerate primers designed according to the conserved regions of published α-tubulin sequences of other fungi on GenBank. The DNA and cDNA full length sequences of α-tubulin gene of T. fuciformis were cloned by SEFA-PCR and RACE，respectively. The DNA sequence of α-tubulin gene of T. fuciformis consisted of 1 962 bp，containing 9 introns and the cDNA sequence consisted of 1 347 bp，coding 448 amino acids. Bioinformatics analysis showed the similarity between α-tubulin cDNAs of T. fuciformis and Cryptococcus neoformans reaches to 80%，the α-tubulin of T. fuciformis belongs to α-tubulin subfamily of Tubulin_FtsZ superfamily and its amino acid sequence contains a conservative GTP binding region GGGTGSG. Phylogenetic tree revealed the evolutionary distance between T. fuciformis and C. neoformans is the nearest.

Key words: Tremella fuciformis, α-tubulin, cloning, sequence analysis, phylogenetic tree