嫁接辣椒对青枯病的抗性及其与渗透调节物质的关系

园艺学报 ›› 2011, Vol. 38 ›› Issue (5): 903-910.

嫁接辣椒对青枯病的抗性及其与渗透调节物质的关系

刘业霞1，姜飞1，张宁1，王洪涛2，艾希珍1,*

（¹山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，园艺作物生物学农业部重点开放实验室，山东泰安 271018；² 山东省枣庄市农业示范园区，山东枣庄 277300）

收稿日期:2011-01-27 修回日期:2011-04-11 出版日期:2011-05-25 发布日期:2011-05-25
通讯作者: 艾希珍

Relationship Between Osmoregulation and Bacterial Wilt Resistance of Grafted Pepper

LIU Ye-xia1，JIANG Fei1，ZHANG Ning1，WANG Hong-tao2，and AI Xi-zhen 1,*

（¹ College of Horticulture Science and Engineering，Shandong Agricultural University，State Key Laboratory of Crop Biology，Tai’an，Shandong 271018，China；² Zaozhuang Agricultural Demonstration Garden，Zaozhuang，Shandong 277300，China）

Received:2011-01-27 Revised:2011-04-11 Online:2011-05-25 Published:2011-05-25
Contact: AI Xi-zhen

摘要/Abstract

摘要： 青枯病是当前设施辣椒主要的土传病害之一。为了选择抗性砧木和探讨嫁接辣椒的抗病机理，通过人工接种青枯病原菌，分析了不同辣椒品种砧木的发病率和病情指数变化，结果表明：‘富根卫士’（简称‘卫士’）发病晚，发病率低，病情指数最小，抗病性最强。以‘卫士’辣椒为砧木，以‘新丰2号’为接穗进行嫁接，以接穗自根嫁接为对照，研究其发病率、病情指数和渗透调节物质的变化。结果表明：嫁接植株的发病率和病情指数显著低于对照，接种20 d时，嫁接植株发病率和病情指数分别比对照低33%和43%。接种前嫁接的植株根系和叶片的含水量、水势、渗透势均低于对照，束缚水/自由水比值、可溶性糖及脯氨酸含量均高于对照。接种后，辣椒根系和叶片的含水量、水势、渗透势均逐渐降低，束缚水/自由水比值逐渐升高，可溶性糖和脯氨酸含量先升高后降低；与对照相比，嫁接植株根系和叶片的含水量、水势、渗透势的降低幅度及束缚水/自由水比值升高幅度明显较小，可溶性糖和脯氨酸含量显著增加。研究结果表明嫁接可显著提高辣椒青枯病的抗性，其抗病机理与渗透调节能力增强有关。

关键词: 辣椒, 砧木, 病情指数, 束缚水, 自由水, 渗透调节

Abstract: Bacterial wilt is one of the primary diseases of pepper grown in solar-greenhouse. In order to select an resistant rootstock and elucidate the resistant mechanism of grafted pepper，changes of disease incidence and disease index of bacterial wilt in different rootstock varieties of pepper were investigated by manual inoculation，with‘Xinfeng 2’as control. The result showed that‘Weishi’was diseased in later days，and showed the lowest disease incidence and disease index，therefore represent the highest disease resistance among the four rootstock varieties and the control. Changes of disease incidence and disease index of bacterial wilt and osmoregulation in grafted（‘Xinfeng 2’scion grafted onto‘Weishi’）and own-root plants（‘Xinfeng 2’，control）of pepper were investigated after being inoculated. The results showed that the disease incidence and disease index of grafted plants were lower than those of control. Measured on 20 d after inoculation，the disease incidence and disease index of grafted plants were lower by 33% and 43% respectively compared with the control. Before inoculation，grafted plants showed lower water content，water potential and osmotic potential，but higher bound water/free water，soluble sugar and proline content in roots and leaves，compared with the control. The water content，water potential and osmotic potential in roots and leaves of pepper plants decreased gradually after inoculation，but bound water/free water increased，the soluble sugar and proline contents increased at early stage，but decreased later. Compared with control plants，the grafted peppers showed less decreasing rates in water content，water potential and osmotic potential，and less increasing rate in bound water/free water. Soluble sugar and proline contents in leaves and roots of grafted peppers were significantly higher than those of control plants. These data indicated that graft significantly increased the bacterial wilt resistance in pepper，and is closely related to the osmoregulation.

Key words: pepper, rootstock, disease index, bound water, free water, osmoregulation

中图分类号:

S 641.3

刘业霞;姜飞;张宁;王洪涛;艾希珍;. 嫁接辣椒对青枯病的抗性及其与渗透调节物质的关系 [J]. 园艺学报, 2011, 38(5): 903-910.

LIU Ye-xia;JIANG Fei;ZHANG Ning;WANG Hong-tao;and AI Xi-zhen;. Relationship Between Osmoregulation and Bacterial Wilt Resistance of Grafted Pepper [J]. ACTA HORTICULTURAE SINICA, 2011, 38(5): 903-910.

[1]	罗海林, 袁雷, 翁华, 闫佳会, 郭青云, 王文清, 马新明. 蚕豆萎蔫病毒2号青海辣椒分离物的鉴定与全基因组序列克隆[J]. 园艺学报, 2023, 50(1): 161-169.
[2]	张晓明, 闫国华, 周宇, 王晶, 段续伟, 吴传宝, 张开春. 甜樱桃砧木新品种‘京春2号’[J]. 园艺学报, 2022, 49(S2): 31-32.
[3]	曹亚从, 张正海, 于海龙, 冯锡刚, 张宝玺, 王立浩. 螺丝椒新品种‘中椒409号’[J]. 园艺学报, 2022, 49(S2): 111-112.
[4]	曹亚从, 张正海, 于海龙, 冯锡刚, 张宝玺, 王立浩. 螺丝椒抗病新品种‘中椒209号’[J]. 园艺学报, 2022, 49(S2): 113-114.
[5]	王永平, 胡明文, 朱文超, 廖芳芳, 白立伟, 高刚. 辣椒新品种‘红辣3号’[J]. 园艺学报, 2022, 49(S2): 115-116.
[6]	刘术均, 惠成章, 赵丽丽, 孙永生, 刘爱群. 中早熟辣椒新品种‘美罗卡’[J]. 园艺学报, 2022, 49(S2): 117-118.
[7]	王飞, 李宁, 尹延旭, 高升华, 徐凯, 姚明华. 辣椒新品种‘鄂椒红元帅’[J]. 园艺学报, 2022, 49(S2): 119-120.
[8]	李宁, 尹延旭, 高升华, 徐凯, 王飞, 姚明华. 辣椒新品种‘锦绣红117’[J]. 园艺学报, 2022, 49(S2): 121-122.
[9]	田红梅, 刘娟, 张长坤, 陶珍, 张建, 王朋成, . 甜瓜砧木用南瓜新品种‘皖砧6号’[J]. 园艺学报, 2022, 49(S2): 127-128.
[10]	方荣, 陈学军, 周坤华, 袁欣捷, 雷刚, 黄月琴. 辣椒新品种‘赣椒18号’[J]. 园艺学报, 2022, 49(S1): 75-76.
[11]	董桑婕, 葛诗蓓, 李岚, 贺丽群, 范飞军, 齐振宇, 喻景权, 周艳虹. 不同光质补光对辣椒幼苗生长、丛枝菌根共生和磷吸收的影响[J]. 园艺学报, 2022, 49(8): 1699-1712.
[12]	聂鑫淼, 栾恒, 冯改利, 王超, 李岩, 魏珉. 硅营养和嫁接砧木对黄瓜幼苗耐冷性的影响[J]. 园艺学报, 2022, 49(8): 1795-1804.
[13]	李颖, 王恒明, 徐小万, 徐晓美, 黄智文. 辣椒新品种‘粤椒8号’[J]. 园艺学报, 2022, 49(8): 1837-1838.
[14]	郑晓东, 袭祥利, 李玉琪, 孙志娟, 马长青, 韩明三, 李少旋, 田义轲, 王彩虹. 油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究[J]. 园艺学报, 2022, 49(7): 1401-1414.
[15]	李平平, 张祥, 刘雨婷, 谢志和, 张芮豪, 赵凯, 吕俊恒, 王梓然, 文锦芬, 邹学校, 邓明华. 辣椒63份种质果皮颜色与呈色物质的关系[J]. 园艺学报, 2022, 49(7): 1589-1601.