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园艺学报 ›› 2011, Vol. 38 ›› Issue (4): 783-789.

• 研究报告 • 上一篇    下一篇

从茶树幼苗中分离茶尺蠖取食诱导的基因

乔金莲1,张娅婷1,朱小佩1,杨会敏1,2,曾 威1,赵 勤1,袁红雨1,*   

  1. 1信阳师范学院生命科学学院,河南信阳 464000;2红河学院生命科学与技术学院,云南蒙自 661100
  • 收稿日期:2011-01-06 修回日期:2011-03-21 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 袁红雨

Identification of Genes Up-regulated by Ectropic obliqua Feeding in Tea Seedlings

QIAO Jin-lian1,ZHANG Ya-ting1,ZHU Xiao-pei1,YANG Hui-min 1,2,ZENG Wei 1,ZHAO Qin1,and YUAN Hong-yu 1,*   

  1. 1 College of Life Science,Xinyang Normal University,Xinyang,He nan 464000,China;2 College of Life Science and Technology,Honghe University,Mengzi,Yunnan 661100,China 
  • Received:2011-01-06 Revised:2011-03-21 Online:2011-04-25 Published:2011-04-25
  • Contact: YUAN Hong-yu1

摘要: 通过抑制差减杂交、差异筛选和实时荧光定量RT-PCR分析,从茶树叶片中分离出19个受茶尺蠖取食诱导的基因,所分离的基因参与茉莉酸(jasmonic acid,JA)的生物合成,细胞壁修饰,次生代谢产物的合成,糖类代谢,病原菌抗性反应,氧化胁迫保护等,其中15个基因在茶树中首次被分离,4个基因在研究植物与昆虫相互作用时首次被发现。研究结果表明茶尺蠖取食激活一系列抗性相关基因,诱导茶树的防御反应。

关键词: 茶树, 茶尺蠖, 昆虫取食, 基因表达

Abstract: Nineteen genes up-regulated by Ectropic obliqua attack from tea plants were isolated by suppression subtractive hybridization(SSH),differential screening,and real-time RT-PCR analysis. These genes were involved in JA synthesis,cell wall modification,secondary metabolism,carbohydrate metabolism,pathogen defensive response and oxidative stress protection. Among these genes,15 clones were firstly isolated from tea plants,and 4 clones firstly found in the studies of molecular mechanism of plant-insect interactions,up to day. The results showed that Ectropic obliqua attack up-regulated defense related genes and induced tea defense responses.

Key words: tea plant, Ectropic obliqua, insect feeding, gene expression

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