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园艺学报 ›› 2011, Vol. 38 ›› Issue (4): 753-760.

• 研究报告 • 上一篇    下一篇

山药珠芽生长过程中激素和糖类物质含量的变化

龙雯虹1,郭华春2,* ,肖关丽3,王 琼2   

  1. 1云南农业大学园林园艺学院,昆明 650201;2云南农业大学薯类作物研究所,昆明 650201;3云南农业大学农学与生物技术学院,昆明 650201
  • 收稿日期:2011-01-25 修回日期:2011-03-30 出版日期:2011-04-25 发布日期:2011-04-25
  • 通讯作者: 郭华春

Variation of Endogenous Hormone and Carbohydrate Contents in Growing Yam Bulbils

LONG Wen-hong1,GUO Hua-chun 2,* ,XIAO Guan-li3,and WANG Qiong2   

  1. 1 College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,China;2 Tuber and Root Crops Research Institute,Yunnan Agricultural University,Kunming 650201,China;3 College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China
  • Received:2011-01-25 Revised:2011-03-30 Online:2011-04-25 Published:2011-04-25
  • Contact: GUO Hua-chun

摘要: 测定了不同生长阶段的山药(Dioscorea opposita)珠芽内源激素IAA、ZR、DHZR、GA3和ABA的含量,以及还原糖、可溶性糖、淀粉和干物质的含量,研究其变化规律。结果表明,珠芽生长期IAA和DHZR含量低,变化小。ZR的含量一直比DHZR高,且变化大,尤其在珠芽生长初期含量下降快。与其它激素相比,GA3含量较高,生长初期呈上升变化,随后缓慢下降,当生长停止时其含量仍较高(1 220.7 ng · g-1)。珠芽完全变褐前,ABA的含量在730.9 ~ 954.9 ng · g-1间波动,在珠芽完全变褐至生长停止期间急剧上升。珠芽开始褐变前,还原糖含量迅速下降,以后变化较小。珠芽生长过程中可溶性糖含量呈曲线上升;淀粉和干物质含量呈直线缓慢上升,当珠芽成熟时可溶性糖、淀粉和干物质含量达到最高。山药珠芽的生长需要高水平的GA3和ZR,当GA3/ABA小于1时,珠芽趋于成熟;茎蔓枯黄珠芽才停止生长,糖、淀粉等物质的积累才终止。

关键词: 山药, 珠芽, 内源激素, 淀粉, 可溶性糖, 还原糖

Abstract: Abstract:Contents of endogenous hormones IAA,ZR,DHZR,GA3,ABA,reducing sugar,soluble sugar,starch and dry matter in yam(Dioscorea opposita)bulbils were tested over developmental cycle. The results showed that concentration of IAA was low and varied gently,so did that of DHZR,while ZR content was higher than DHZR content throughout bulbil developing stage and changed more sharply especially at early stage. The content of GA3,compared with other hormone contents,was very high throughout bulbil developing stage,it maintained 1 220.7 ng · g-1 at last,though it began to reduce after bulbils became brown. The content of ABA fluctuated from 730.9 ng · g-1 to 954.9 ng · g-1 before bulbils became brown completely,then increased sharply. The content of reducing sugar reduced rapidly at first and changed little after bulbil browning. The content of soluble sugar curve rose,while the contents of starch and dry matter rose linearly. When bulbils were mature,the contents of soluble sugar,starch and dry matter reached the peak. The results indicated that high GA3 and ZR content were necessary for bulbil development,and bulbils would to be mature after the ratio of GA3 to ABA was less than 1. As the result of endogenous hormone regulation,matters such as reducing sugar,soluble sugar,starch and dry matter continued to accumulate until the bulbils matured and the vines on which bulbils grown were yellow and dry.

Key words: Dioscorea opposita, bulbil, endogenous hormone, starch, soluble sugar, reducing sugar

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