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园艺学报 ›› 2011, Vol. 38 ›› Issue (3): 563-570.

• 研究报告 • 上一篇    下一篇

唐菖蒲茉莉酸生物合成关键酶基因LOX1的克隆及表达分析

连青龙,辛海波,张自由,钟雄辉,罗 弦,义鸣放*   

  1. 中国农业大学观赏园艺与园林系,北京 100193
  • 收稿日期:2010-08-11 修回日期:2011-01-11 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 义鸣放

Cloning and Expression Analysis of Lipoxygenase-1 Gene Encoding a Key Enzyme of the Biosynthesis of Jasmonic Acid from Gladiolus hybridus

LIAN Qing-long,XIN Hai-bo,ZHANG Zi-you,ZHONG Xiong-hui,LUO Xian,and YI Ming-fang*   

  1. Department of Ornamental Horticulture and Landscape Architecture,China Agricultural University,Beijing 100193,China
  • Received:2010-08-11 Revised:2011-01-11 Online:2011-03-25 Published:2011-03-25
  • Contact: YI Ming-fang

摘要: 以唐菖蒲(Gladiolus hybridus)品种‘Rose Supreme’的球茎为试材,通过RT-PCR和RACE技术克隆到了一个全长为2 797 bp的茉莉酸(Jasmonic acid,JA)生物合成关键酶LOX基因的cDNA序列,命名为GhLOX1,属于9-LOX。该序列含有一个2 541 bp的开放阅读框(ORF),编码846个氨基酸,推导的蛋白质分子量为94.90 kD。RT-PCR表达分析表明,该基因在唐菖蒲叶、花、根、匍匐茎、新球茎和籽球上都表达,而在叶和匍匐茎中表达量最高,在花和籽球中表达量较低;离体条件下,经过0、0.1、0.5、1.0、2.0 mmol · L-1的不同浓度梯度的水杨酸(SA)处理后,其表达水平随着浓度的升高而降低,当SA浓度达到2.0 mmol · L-1时没有表达。

关键词: 唐菖蒲, LOX, JA, 克隆, 表达

Abstract: A full length cDNA named GhLOX1 belonging to 9-LOX was cloned in Gladiolus hybridus‘Rose Supreme’corms by RT-PCR and RACE. The open reading frame encompassed 2 541 bp encoding a polypeptide of 846 amino acids with calculated protein molecular mass of 94.90 kD. RT-PCR analysis showed that GhLOX1 gene was expressed in leaf,flower,root,stolon,corm,cormel,and higher in the leaf and stolon. In vitro,with the different concentration of salicylic acid(SA)0,0.1,0.5,1.0,2.0 mmol · L-1 treatment respectively,the expression level decreased as the concentration increased. LOX1 wasn’t expressed after treating 2.0 mmol · L-1 SA.

Key words: Gladiolus hybridus, lipoxygenase, jasmonic acid, cloning, expression

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