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园艺学报 ›› 2001, Vol. 28 ›› Issue (2): 167-169.

• 研究报告 • 上一篇    下一篇

柑桔MADS盒APETALA1同源DNA 片段克隆

林伯年;刘春铃;徐昌杰;陈大明
  

  1. (浙江大学园艺系, 杭州310029)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-04-25 发布日期:2001-04-25

Cloning DNA Fragment of APETALA1 Homologue of the Citrus MADS-box

Lin Bonian, Liu Chunling, Xu Changjie, and Chen Daming   

  1. (Horticultur al Department , Zhejiang University , Hangzhou 310029)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-04-25 Published:2001-04-25

摘要: 用PCR 扩增法, 从柑桔基因组分离出了AP 1 同源基因中的一个片段。序列分析表明, 它与其它植物的MADS 盒基因同源性较高, 氨基酸序列同源45%~73%, 但是否与发育有关, 还需做功能性检测。

关键词: 柑桔, MADS 盒基因, PCR

Abstract: Through PCR method, a fragment of AP1 homologue is separated from citrus gene library of DNA. This fragment is conserved withMADS􀀁box genes in other plants. The amino acid sequence deduced from the exons is 45%-
73% homologous to that of the corresponding regions of MADS􀀁box genes in other plants. This fragment is appeared at GenBank. Serial number is AF 2639911.

Key words: Citrus, MADS-box genes, PCR

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