金合欢组织培养和快速繁殖的研究

园艺学报 ›› 2001, Vol. 28 ›› Issue (2): 149-152.

金合欢组织培养和快速繁殖的研究

翟建中¹；顾梅俏¹；程荣昌²；张　燕²；刘小军²

(¹ 上海市花卉育种中心, 上海201615 ;　²上海松江花桥现代农业公司, 上海201601)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-04-25 发布日期:2001-04-25

Tissue Culture and Rapid Propagation of Acacia dealbata

Zhai Jianzhong¹, Gu Meiqiao¹, Chen Rongchang², Zhang Yan², and Liu Xiaojun²

(¹ Shanghai Flower Breeding Center , Shanghai 201615;　² Shanghai Songjiang Rose Bridge Modern Agriculture Company Limited , Shanghai 201601)

Received:1900-01-01 Revised:1900-01-01 Online:2001-04-25 Published:2001-04-25

摘要/Abstract

摘要： 采用金合欢(Acacia dealbata) 大树顶芽、幼苗茎尖、种子小苗上胚轴和幼叶作外植体进行组培和快繁研究。结果表明: 用MS + 6-BA 6 mg/L + NAA 0. 1 mg/L + NaH₂PO₄ 30 mg/L 可提高愈伤组织的出芽率, 有利于扩大繁殖系数, 而用MS + 6-BA 2 mg/L + NAA 0. 1 mg/L + NaH₂PO₄ 30 mg/L 可以增加发枝率。珍珠岩+ 泥炭(1∶1) 基质可促进小苗后期生长,加速成苗。

关键词: 金合欢, 组织培养, 快速繁殖, 炼苗

Abstract: Acacia dealbata is a famous kind of economical and oremental trees in Australia. In this test , shoot tips taken from big trees and seedlings , little leaves from young plants , epicotyls from seeds were collected as explants that were studied on tissue culture and rapid propagation. The results
showed that all four kinds of explants have been induced callus formation. Buds and shoots can be induced on callus except little leaf’s callus in morphogensis. The medium MS + 6-BA 6 mg/ L + NAA 0. 1 mg/ L + NaH₂PO₄30 mg/ L may increase sprouting bud rate from callus. However , the medium
MS + 6-BA 2 mg/ L + NAA 0. 1 mg/ L + NaH₂PO₄ 30 mg/ L may increase shoot growing rate. Plantlets from test tubes grew better on the growth medium perlite + peat (1∶1) than on other components in acclimatization stage.

Key words: Acacia dealbata, Tissue culture, Rapid propagation, Acclimatization

中图分类号:

S 68
Q 813

翟建中;顾梅俏;程荣昌;张　燕;刘小军. 金合欢组织培养和快速繁殖的研究[J]. 园艺学报, 2001, 28(2): 149-152.

Zhai Jianzhong;Gu Meiqiao;Chen Rongchang;Zhang Yan;and Liu Xiaojun. Tissue Culture and Rapid Propagation of Acacia dealbata[J]. ACTA HORTICULTURAE SINICA, 2001, 28(2): 149-152.

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