强光胁迫下外源NO对霍山石斛叶绿素荧光和抗氧化系统的影响

园艺学报 ›› 2008, Vol. 35 ›› Issue (8): 1215-1220.

强光胁迫下外源NO对霍山石斛叶绿素荧光和抗氧化系统的影响

樊洪泓¹ ;李廷春^1,2 ;李正鹏^1,3;林毅^1*;蔡永萍¹

(¹安徽农业大学生命科学学院, 合肥 230036;²安徽省烟草研究所, 合肥 230031; ³安徽科技学院, 凤阳 233100)

收稿日期:2008-03-03 修回日期:2008-07-08 出版日期:2008-08-25 发布日期:2008-08-25
通讯作者: 林毅

Effects of Exogenous Nitric Oxide on The Chlorophyll Fluorescence and Antioxidant System of Dendrobium huoshanense Under High Light Stress

FAN Hong-hong¹, LI Ting-chun², LI Zheng-peng^1,3, LIN Yi^1*, and CAI Yong-ping¹

(¹School of Life Science, Anhui Agriculture University, Heifei 230036, China ; ² Anhui Provincial Tobacco Research Institute, Hefei 230031, China ; ³School of Life Science , Anhui Science and Technology university, Fengyang Anhui 233100, China )

Received:2008-03-03 Revised:2008-07-08 Online:2008-08-25 Published:2008-08-25
Contact: LIN Yi

摘要/Abstract

摘要： 以兼性景天酸代谢(CAM)植物霍山石斛为材料，研究强光胁迫条件下，外源NO对其叶绿素荧光和抗氧化系统的影响。结果表明： 0. 1 mmol·L^-1硝普钠(SNP)处理提高了石斛光合系统Ⅱ(PSⅡ)的光能转换效率和潜在活性，增加了过剩光能的非光化学耗散，缓解了光抑制的发生，同时通过增强抗氧化系统的活性氧清除能力，有效保护了光合机构免受强光胁迫的伤害，PSⅡ反应中心得以较快恢复。而经0.5 mmol·L^-1SNP处理后，霍山石斛的光能转换系统未能通过有效的光能转换和非光化学反应耗散过剩的光能，降低了抗氧化系统中SOD、POD和CAT的活性，加剧了PSⅡ反应中心光抑制的发生。

关键词: 霍山石斛, NO, 强光胁迫, PSⅡ

Abstract: In this paper, the effects of NO donor sodium nitroprusside (SNP) on the chlorophyll fluorescence and antioxidant system of Dendrobium huoshanense under high light stress were studied. The result showed that the efficiency of light energy conversion and nonphotochemical dissipation of excess light energy for photosystemⅡ were inproved, and the capacity of scavenging active oxygen for antioxidant system was increased as well by presoaking with 0.1 mmol·L^-1SNP , which alleviated the photoinhibition on leaves of Dendrobium huoshanense significantly. Therefore the PSⅡ was protected efficiently and the recovery was faster. On the contrary, the leaves presoaked in 0.5 mmol·L^-1SNP were severely inhibited by the high light and the enzyme activities of SOD, POD and CAT were decreased.

Key words: Dendrobium huoshanense, NO, high light stress, PSⅡ

中图分类号:

S 682.31

樊洪泓;李廷春;;李正鹏;林毅;蔡永萍. 强光胁迫下外源NO对霍山石斛叶绿素荧光和抗氧化系统的影响[J]. 园艺学报, 2008, 35(8): 1215-1220.

FAN Hong-hong;LI Ting-chun;LI Zheng-peng;LIN Yi;and CAI Yong-ping. Effects of Exogenous Nitric Oxide on The Chlorophyll Fluorescence and Antioxidant System of Dendrobium huoshanense Under High Light Stress[J]. ACTA HORTICULTURAE SINICA, 2008, 35(8): 1215-1220.

[1]	戴亚峰，王诗文，李诚，黄跃华，张恩亮. 霍山石斛新品种‘九仙尊2号’[J]. 园艺学报, 2020, 47(1): 201-202.
[2]	芮文婧1，王晓敏1,2,3,，张倩男1，胡学义3，胡新华4，付金军4，高艳明1,2,3，李建设1,2,3,. 番茄353份种质资源表型性状遗传多样性分析[J]. 园艺学报, 2018, 45(3): 561-570.
[3]	王芬，田歌，刘晶晶，于波，葛顺峰，姜远茂. 硼对平邑甜茶幼苗硝态氮吸收、利用及分配特性的影响[J]. 园艺学报, 2017, 44(7): 1244-1250.
[4]	李国亮，钱伟，张淑江，李菲，章时藩，张慧，谢露露，武剑，王晓武，孙日飞**. 白菜翻译起始因子eIF(iso)4E.a/c 与芜菁花叶病毒TuMV-C4/UK1 蛋白互作分析[J]. 园艺学报, 2017, 44(7): 1299-1308.
[5]	李陈静，牛建新*，裴茂松，曹福军，全绍文. 库尔勒香梨9个新miRNA克隆鉴定[J]. 园艺学报, 2016, 43(9): 1803-1815.
[6]	孙德智，韩晓日，彭靖，范富. 外源水杨酸对NaCl胁迫下番茄幼苗PSⅡ光化学效率及光能分配利用的影响[J]. 园艺学报, 2016, 43(8): 1482-1492.
[7]	张少平1,2，洪建基1，邱珊莲1,*，郑云云1,2，张帅1，吴松海1，何炎森1，郑开斌1,2，郑加协1. 紫背天葵高通量转录组测序分析[J]. 园艺学报, 2016, 43(5): 935-946.
[8]	束胜;孙锦;郭世荣;李娟;刘超杰;王长义;杜长霞. 外源腐胺对盐胁迫下黄瓜幼苗叶片PSⅡ光化学特性和体内离子分布的影响[J]. 园艺学报, 2010, 37(7): 1065-1072.
[9]	杨凤娟;魏珉;苏秀荣;王秀峰;. 不同浓度NO₃^-胁迫下黄瓜幼苗根系分生区细胞内Ca²⁺分布变化的差异[J]. 园艺学报, 2009, 36(9): 1291-1298.
[10]	孙永平;张治平;徐呈祥;申春苗;高　充;汪良驹. 5- 氨基乙酰丙酸处理对低温下西瓜叶片快速叶绿素荧光诱导曲线的影响[J]. 园艺学报, 2009, 36(5): 671-678.
[11]	廖伟彪;张美玲;吴永华;肖洪浪. 一氧化氮和过氧化氢对地被菊扦插生根的影响[J]. 园艺学报, 2009, 36(11): 1643-1650.
[12]	金青;马绍鋆;蔡永萍;何金铃;林毅;皮瑞. 霍山石斛类原球茎诱导及其发育过程研究[J]. 园艺学报, 2009, 36(10): 1525-1530.
[13]	徐慧妮;王秀峰;孙旭东;杨凤娟;杜栋良. 黄瓜促分裂原活化蛋白激酶基因的生物信息学分析及原核表达[J]. 园艺学报, 2008, 35(7): 1017-1022.
[14]	朱丽琴;朱树华;周杰. NO延缓园艺产品成熟和衰老的生理效应研究进展[J]. 园艺学报, 2008, 35(10): 1539-1544.
[15]	李华云;庄军平;黄胜琴;叶庆生. 高浓度CO₂ 对蝴蝶兰CO₂ 吸收速率和生长的影响[J]. 园艺学报, 2007, 34(3): 705-710.