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园艺学报 ›› 2001, Vol. 28 ›› Issue (1): 19-24.

• 研究论文 • 上一篇    下一篇

果用香蕉薄片外植体植株再生的研究 

黄 霞;黄学林*;王鸿鹤;李筱菊   

  1. (中山大学生命科学学院, 广州510275)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-02-25 发布日期:2001-02-25

Studies on the Plant Regeneration from the Micro-Cross Sections ofBanana

Huang Xia, Huang Xuelin, Wang Honghe, and Li Xiaoju   

  1. (School of Life Sciences , Zhongshan University , Guangzhou 510275)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-02-25 Published:2001-02-25

摘要: 利用薄片培养方法, 通过直接和间接器官发生途径分别获得了‘广东631’香蕉的再生植株。该途径受多种因素影响。通过正交试验和单因子试验确定了最佳培养条件: (1)直接器官发生培养基为Ma (MS modified) + BAP 10μmol/L + KT 50μmol/L + IAA 1μmol/L ; (2)
愈伤组织诱导培养基为Mb (B5 modified) + Dicamba 10μmol/L + IAA 1μmol/L + 2 ,4-D 0. 7μmol/L + NAA 0. 5μmol/L + BAP 4. 4μmol/L + 活性炭1 g/L ; (3) 愈伤组织继代培养基为Mb + Dicam-ba 5μmol/L + IAA 1μmol/L + 2 ,4-D 0. 4μmol/L + BAP 22μmol/L + KNO3 500 mg/L + 维生素B1 40 mg/L + 活性炭0. 5 g/L ; (4) 分化培养基为1/ 2 Ma + BAP 1μmol/L + IAA 0. 5μmol/L + 活性炭1 g/L ; (5) 成苗培养基为Ma + BAP 1μmol/L + KT 4. 6μmol/L + NAA 1μmol/L。愈伤组织诱导和继代培养需在黑暗中进行。

关键词: 香蕉, 薄片培养, 器官发生

Abstract: The organogenesis of ‘Guangdong 631’banana by micro-cross sections culture and the factors influencing plant regeneration were investigated. The optimal culture condition was established by a statistical method and making research on the single factor : (1) the medium for direct organogenesis contained Ma (MS modified) , BAP 10μmol/ L , KT 50μmol/ L and IAA 1μmol/ L ;
(2) the medium for callus induction contained Mb (B5 modified) , dicamba 10μmol/ L , IAA 1μmol/L , 2 , 4-D 0. 7μmol/ L , NAA 0. 5μmol/ L , BAP 4. 4μmol/ L and 1 g/ L activated charcoal ; (3) the medium for callus subculture contained Mb , 5μmol/ L dicamba , IAA 1μmol/ L , 2 ,4-D 0. 4μmol/ L , BAP 22μmol/ L , KNO3 500 mg/ L , vitamine 40 mg/ L B1 and activated 0. 5μg/L charcoal ; (4) the medium for induction shoot buds from the callus was composed of halfstrength Ma , BAP 1μmol/ L , 0. 5μmol/ L IAA and 1 g/ L activated charcoal ; (5) the plant regeneration medium was composed of Ma , BAP 1μmol/ L , KT 4. 6μmol/ L and NAA 1μmol/ L. The callus induction and subculture was kept in darkness. The application of micro-cross sections of
banana for regeneration of plants might be a useful alternative to cell suspensions and protoplast culture in banana transformation.

Key words: Banana, Micro-cross sections culture, Organogenesis

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