https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2002, Vol. 29 ›› Issue (6): 579-580.

• 研究报告 • 上一篇    下一篇

结球甘蓝cDNA文库的构建和鉴定

曹必好1,2;雷建军1,2;宋洪元1;宋 明1;杨朝辉1;Chengbin Xiang3;David. J . Oliver3;李淡琼1   

  1. (1 西南农业大学园艺系, 重庆400716 ; 2 华南农业大学园艺系, 广州510642 ; 3 美国依阿华州立大学, 依阿华州50011)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-12-25 发布日期:2002-12-25

cDNA Library Construction and Identification of Cabbage

Cao Bihao1,2, Lei Jianjun1,2, Song Hongyuan1, Song Ming1, Yang Zhaohui1, Chengbin Xiang3, David. J . Oliver3,and Li Danqiong1   

  1. (1 Department of Horticulture , South2west Agricultural University , Chongqing 400716 , China ; 2 Department of Horticueture , South2China Agricultural University , Guangzhou 510642 , China ; 3 Iowa state university , 50011 America)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-12-25 Published:2002-12-25

摘要: 构建了结球甘蓝cDNA 文库。文库的宿主菌为E. coli XL1-lue。对文库的部分特性进行研究,滴度为2 ×108~4 ×108 pfu/ mL , 插入片段的大小均在500 bp 以上, 以1kb 以上的片段占大多数, 重组体的重组率为95 % , 用抗病基因片段作探针进行噬菌体原位杂交, 杂交信号明显。对文库进行了扩增、保存。

关键词: 甘蓝, 结球甘蓝, cDNA 文库

Abstract: A cDNA library of cabbage was constructed and an expression plasmid introduced into E. coli XL12Blue was used as cloning vector. The titer of cDNA library is 2 ×108 - 4 ×108 pfu/ mL ; most of the fragments are more than 500bp , the recombinant rate of the library is about 95 %. Probed with resistant gene fragment , phage in situ hybridization , the hybridization signal is clear , which shows this library is good , and the library had been amplified and stored.

Key words: Cabbage, cDNA library

中图分类号: