核桃早实性状的RAPD分析

园艺学报 ›› 2002, Vol. 29 ›› Issue (6): 573-574.

核桃早实性状的RAPD分析

杨克强¹;王跃进¹;张银东²;郑学勤²

(¹ 西北农林科技大学园艺学院, 杨凌712100; ² 热带作物分子生物学国家重点实验室, 海口571101)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-12-25 发布日期:2002-12-25

RAPD Analysis for the Identification of the Precocious Trait in Walnuts

Yang Keqiang¹, Wang Yuejin¹, Zhang Yindong², and Zheng Xueqin²

(¹Horticultur al College, Northwester n Sci-tech University of Agriculture a nd Forestry, Yangling 712100, China ; ²National Key Biotechnology Laboratory f or Tropical Crops, Haikou 571101, China )

Received:1900-01-01 Revised:1900-01-01 Online:2002-12-25 Published:2002-12-25

摘要/Abstract

摘要： 用BSA (Bulked Segregant Analysis) 法获得了与核桃早实性状相关的RAPD 标记OPB208₉₀₀。混合分离群体的混合样来自早实品种辽1 的自然授粉实生后代。用220 个随机引物扩增早实和晚实混合样, 只有引物OPB208 ( 5. GTCCACACGG 3. ) 在早实混合样及其个体上扩增出了约900 bp 的特异带OPB208₉₀₀, 而在晚实混合样及其个体上无此特异带。用OPB208 为引物, 以19 个品种和3 个株系的DNA 为模板进行扩增,特异带OPB208₉₀₀在17 个早实品种中的12 个个体上出现, 而5 个晚实品种( 系) 上均无此带。

关键词: RAPD, 早实性状, 核桃

Abstract: A random amplified polymorphic DNA (RAPD) marker linked to the precocious trait of walnut (Juglans regia L. ) , OPB208900, was identified with bulked segregant analysis ( BSA) . Two bulks of DNA samples were created from open2pollinated Liao 1 (an precocious cultivar) . The bulks were screened using 220 random primers (OP Kit) . OPB208 ( 5. GTCCA CACGG 3. ) generated a specific fragment with precocious individuals and without backward individuals. The specific fragment of approximately 900 base pairs ( bp) , was designated as OPB208₉₀₀. 22 DNAs from 19 cultivars and 3 clones were used as templates for RAPD analysis with primer OPB208 respectively. The specific band of OPB208₉₀₀ was present in 12 out of 17 precocious cultivars and absent in 5 backward cultivars/ clones. The RAPD marker OPB208₉₀₀ is related with precocious trait in walnut and
would be used to distinguish the precocious trait and backward trait in walnut.

Key words: RAPD, Precocious trait, Walnut (Juglans regia L.)

中图分类号:

S 664. 1

杨克强;王跃进;张银东;郑学勤. 核桃早实性状的RAPD分析[J]. 园艺学报, 2002, 29(6): 573-574.

Yang Keqiang;Wang Yuejin;Zhang Yindong;and Zheng Xueqin. RAPD Analysis for the Identification of the Precocious Trait in Walnuts[J]. ACTA HORTICULTURAE SINICA, 2002, 29(6): 573-574.

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