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园艺学报 ›› 2002, Vol. 29 ›› Issue (6): 547-550.

• 研究论文 • 上一篇    下一篇

硒对菠菜抗氧化系统及过氧化氢含量的影响

李登超1,2;朱祝军1,3;徐志豪3
  

  1. (1 浙江大学园艺系, 杭州310029 ; 2 浙江林学院科技处, 临安311300 ; 3 浙江省农业科学院园艺研究所, 杭州310021)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-12-25 发布日期:2002-12-25

Effects of Selenium on Antioxidative System and H2O2 Content in Spinach

Li Dengchao1,2, Zhu Zhujun1, and Xu Zhihao3
  

  1. (1 Department of Horticulture , Zhejiang University , Hangzhou 310029 , China ; 2 Zhejiang Forestry College , Lin’an 311300 , China ; 3 Institute of Horticulture , Zhejiang Academy of Agricultural Sciences , Hangzhou 310021 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-12-25 Published:2002-12-25

摘要: 研究了不同浓度硒对菠菜的生长、超氧化物歧化酶(SOD) 、抗坏血酸过氧化物酶(APX) 、过
氧化氢酶(CAT) 、脱氢抗坏血酸还原酶(DHAR) 、谷胱甘肽过氧化物酶(GSH-PX) 等酶活性以及抗坏血酸(AsA) 、过氧化氢(H2O2) 含量的影响。结果表明: 低浓度硒( ≤0. 1 mg·L-1) 促进了菠菜的生长, 而高浓度硒(1. 0 mg·L-1) 抑制生长; 低浓度硒提高了SOD、CAT及GSH2PX活性, 而APX和DHAR 活性则随硒浓度的升高而降低; AsA 含量随硒浓度的增加而减少, H2O2 含量先随硒浓度的增加而下降, 但在高硒浓度时又上升。总之, 适量的硒处理能增强菠菜的抗氧化能力, 增加产量。

关键词: 菠菜, 硒, 抗氧化作用, 抗坏血酸, 过氧化氢

Abstract: The effects of different concentrations of seleniumon the growth , the activities of superoxide dismutase (SOD) , catalase (CAT) , ascorbate peroxidase (APX) , dehydroascorbate reductase (DHAR) , glutathione
peroxidase (GSH2PX) and the content of ascorbate (AsA) , hydrogen peroxide (H2O2) were studied in the hydroponic spinach. The results showed that the growth was enhanced by selenium at the low level ( ≤0. 1 mg·L-1) , but inhibited at the high level (1. 0 mg·L-1) . The activities of SOD , CAT and GSH2PX were increased and the activities of APX and DHAR were decreased. The contents of AsA and H2O2 decreased with the increasing selenite concentration , whereas , H2O2 content decreased firstly and then increased. From our results , we conclude that proper selenite concentration could increase the yield and antioxidative activity of spinach.

Key words: Spinach, Selenium, Antioxidative activity, Ascorbate, Hydrogen peroxide

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