https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2002, Vol. 29 ›› Issue (5): 493-494.

• 研究报告 • 上一篇    下一篇

非洲菊试管苗叶片的组培快繁 

徐士清;杨世湖*;倪 丹;万建民
  

  1. (南京农业大学作物遗传与种质创新国家重点实验室, 江苏省植物基因工程研究中心, 南京210095)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-25 发布日期:2002-10-25

In Vitro Micropropagation of Gerbera Leaf

Xu Shiqing, Yang Shihu*, Ni Dan, and Wan Jianmin   

  1. (National Key Laboratory for Crop Genetics and Germplasm Enhancement , Nanjing Agricultural University , Nanjing 210095 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-25 Published:2002-10-25

摘要: 用非洲菊试管苗叶片作外植体获得了有再分化能力的愈伤组织和正常的再生植株。在适宜培
养基上叶片切块的出愈率和出芽率最高可达96 %和90 %; 小苗4 周增殖倍数为10. 85 , 生根率99 % , 最快4周就能从叶片切块成苗。试管苗叶片快繁最适培养基为: 起始MS + 62BA 3. 0 mg·L-1 + NAA 0. 1 mg·L-1; 增殖MS + 62BA 3. 0 mg·L-1 + NAA 0. 2 mg·L-1; 生根1/ 2 MS + IAA 1. 0 mg·L-1 。用作外植体的试管苗以3~4叶期为佳, 叶片切块以4 mm ×4 mm为宜。

关键词: 非洲菊, 叶片, 组织培养, 快速繁殖

Abstract: Regenerable calli and plantlets were obtained from leaf and petiole segments of Gerbera in vitro plantlets and those regenerated plantlets were morphologically normal . The rate of callus formation and rate of shoot
formation on optimum medium were as high as 96 % and 90 % separately. Four weeks’in vitro multiple times for those plantlets was 10. 85 while root formation rate was 99 % during root inducing. The earliest plantlets could be
obtained in 4 weeks. Optimal media for micropropagation after optimization experiments were MS + 62BA 3. 0 mg·L-1 + NAA 0. 1 mg·L-1 for induction ; MS + 62BA 3. 0 mg·L + NAA 0. 2 mg·L-1 for multiplication of plantlets ;1/ 2 MS + IAA 1. 0 mg·L-1 for root formation. In vitro plantlets used for explants was at the stage with 3 - 4 leaves and suitable size of cut leaf segments was about 4 mm ×4 mm.

Key words: Gerbera, Leaf, Tissue culture, Micropropagation

中图分类号: