蝴蝶兰花梗节间段培养繁殖的初步研究

园艺学报 ›› 2002, Vol. 29 ›› Issue (5): 491-492.

蝴蝶兰花梗节间段培养繁殖的初步研究

鲁雪华¹;郭文杰¹;徐立晖²;林新华²

(¹ 福建省农业科学院生物技术中心, 福州350003 ;　²福建省罗源县花卉种苗基地, 罗源350600)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-25 发布日期:2002-10-25

The Preliminary Research on the Rapid Propagation of Phalaenopsis by Usingthe Joint-points of Flower-stems as Explants

Lu Xuehua¹, Guo Wenjie¹, Xu Lihui², and Lin Xinhua²

(¹ Biotechnological center , Fujian Academy of Agricultural sciences , Fuzhou 350003 , China ;² Fujian Luoyuan County Flower Seedling Base , Luoyuan 350600 )

Received:1900-01-01 Revised:1900-01-01 Online:2002-10-25 Published:2002-10-25

摘要/Abstract

摘要： 采用蝴蝶兰植株的茎尖、根尖、花梗节、花梗节间切段作外植体诱导类原球茎, 其中花梗节
间切段诱导频率明显高于其它外植体, 用改良的MS培养基+ 6-BA 5～7. 5 mg/L + NAA 0. 5 ～1. 0 mg/L + 椰乳汁15 % 可加速类原球茎的形成。选用水藓作为试管苗的移栽基质有利于提高小苗的成活率。

关键词: 蝴蝶兰, 组织培养, 花梗节间段, 类原球茎, 基质

Abstract: The stem-tip , root-tip , flower-stem-knot and cutting segment of lower-stem-knot of the plant were used as explants to study the rapid propagation method of Phalaenopsis schilleriana by tissue culture. The result indicated that the explants from different parts of the plants were different in protocorm like body induction rate. Among all the explants , the cutting section of flower-stem-knot was fastest in inducing the portocorm like body. The formation rate of protocorm like body could be accelerated by using the improved MS medium supplemented with 6-BA 5 - 7. 5 mg/ L , NAA 0. 5 - 1. 0 mg/ L + 15 %CW. It is beneficial to improving the survival rate to take the
Sphagnum moss as the growing base of the tube seedlings in transplanting.

Key words: Phalaenopsis, Flower-stem-joint-points, Protocorm like-body, Base

中图分类号:

S 68

鲁雪华;郭文杰;徐立晖;林新华. 蝴蝶兰花梗节间段培养繁殖的初步研究[J]. 园艺学报, 2002, 29(5): 491-492.

Lu Xuehua;Guo Wenjie;Xu Lihui;and Lin Xinhua. The Preliminary Research on the Rapid Propagation of Phalaenopsis by Usingthe Joint-points of Flower-stems as Explants[J]. ACTA HORTICULTURAE SINICA, 2002, 29(5): 491-492.

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