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园艺学报 ›› 2005, Vol. 32 ›› Issue (4): 738-740.

• 研究报告 • 上一篇    下一篇

桂花离体培养与快速繁殖技术的初步研究

宋会访1;葛 红2;周 媛1;王彩云1   

  1. 1 华中农业大学园艺林学学院, 武汉430070; 2 中国农业科学院蔬菜花卉研究所, 北京100081
  • 收稿日期:2004-09-28 修回日期:2004-11-15 出版日期:2005-08-25 发布日期:2005-08-25

Primary Study on in Vitro Culture and Micropropagation of Sweet Osmanthus

Song Huifang1;Ge Hong2;Zhou Yuan1;Wang Caiyun1   

  1. 1College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China; 2 Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
  • Received:2004-09-28 Revised:2004-11-15 Online:2005-08-25 Published:2005-08-25

摘要: 以桂花(Osmanthus fragrans Lour. ) 胚和新梢茎段为外植体进行了离体培养与快速繁殖研究,筛选出最适培养基——— (1) 胚萌发: MS +BA 1.00 mg·L - 1 +蔗糖3%; ( 2) 新梢茎段启动培养: LMc +KT 8.00 mg·L - 1+NAA 0.10 mg·L - 1 +蔗糖3%; ( 3) 继代增殖培养: LMc + TDZ 0.50 mg·L - 1 +NAA0.10 mg·L - 1 +蔗糖3%; (4) 生根培养: 1/2MS +NAA 2.00 mg·L - 1 +蔗糖3%。采用腐殖质土为栽培基质, 移栽成活率可达80%以上。

关键词: 桂花, 离体培养, 快速繁殖

Abstract: Using the embryo, stem-segment of new shoots as the exp lants, in vitro culture and high frequency propagation of sweet osmanthus were studied. The results showed that the optimizing media for various stages were as follows: (1) Initial medium for embryo: MS +BA 1.00 mg·L - 1 + 3% sucrose; (2) Initial
medium of stem-segment for new shoots: LMc + KT 8.00 mg·L - 1 +NAA 0.10 mg·L - 1 + 3% sucrose; (3) Clump shoot regeneration medium: LMc + TDZ 0.50 mg·L - 1 +NAA 0.10 mg·L - 1 + 3% sucrose; ( 4) Rooting medium: 1 /2MS +NAA 2.00 mg·L - 1 + 3% sucrose. Using humus soil as culture substrate, the
survival percentage of plantlets could reach 80%.

Key words: Sweet osmanthus, In vitro culture, Rapid propagation

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