https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2002, Vol. 29 ›› Issue (5): 438-442.

• 研究论文 • 上一篇    下一篇

大白菜3号、6号染色体双三体及其初级三体的鉴定

申书兴1;李振秋2;张成合1;王彦华1;陈雪平1   

  1. (1 河北农业大学园艺学院, 保定071001 ; 2 河北大学生命科学学院, 保定071001)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-10-25 发布日期:2002-10-25

Identification of Double Triplo-3, 6 and Acquisition of Primary Triplo-3 andTriplo-6 in Chinese Cabbage

Shen Shuxing1, Li Zhenqiu2, Zhang Chenghe1, Wang Yanhua1, and Chen Xueping1   

  1. (1 Departerment of Horticulture , Hebei Agricultural University , Baoding 071001 , China ; 2 College of Life Science , Hebei University , Baoding 071001 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-25 Published:2002-10-25

摘要: 利用有丝分裂染色体核型分析的方法, 鉴定同源四倍体大白菜小孢子株系940521为3 号、6 号染色体双三体; 在其与冠291 杂交的F1代中, 利用减数分裂中期Ⅰ染色体观察的方法, 鉴定出了3 号染色体三体和6号染色体三体, 并对F1代中的两种三体及双三体的数目进行统计, 计算出双三体中的3 号、6 号额外染色体的雌配子传递率分别为28. 26 %和47. 83 %; 利用TTC 染色方法测定3 号染色体三体、6 号染色体三体及双三体的花粉生活力, 结果表明: 3 号染色体三体、6 号染色体三体及双三体的花粉生活力差异达极显著水平。

关键词: 大白菜, 染色体, 初级三体, 双三体

Abstract: An autotetraploid Chinese cabbage 〔Brassica campestris ssp. pekinensis (Rupr.) Olsson〕microspore line 940521 was designated as double triplo23 , 6 by mitotic chromosome karyotype analysis. By meiosis metaphase Ⅰchromosome observation , triplo23 , triplo26 and double triplo23 , 6 were identified in the F1 of line 940521 ×Guan 291. The transmission of extra chromosomes in double triplo23 , 6 was calculated and the result
showed that the rates of No. 3 and No. 6 chromosome were 28. 26 % and 47. 83 % respectively. The pollens of triplo23 , triplo26 and double triplo23 , 6 were stained with TTC and their activities were measured. The result
showed that the differences of pollen activity among triplo23 , triplo26 and double triplo23 , 6 were significant at 0. 01 level .

Key words: Chinese cabbage, Chromosome, Primary trisomic, Double trisomics

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