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园艺学报 ›› 2002, Vol. 29 ›› Issue (4): 348-352.

• 研究论文 • 上一篇    下一篇

大白菜子叶离体培养再生植株

张凤兰1;高 田2;徐家炳1
  

  1. ( 1 北京市农林科学院蔬菜研究中心, 北京100089; 2 日本国立岩手大学农学部, 盛冈020􀀁8550)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-08-25 发布日期:2002-08-25

Plant Regeneration from Cotyledonary Explants of Chinese Cabbage Cultured in Vitro

Zhang Fenglan1, Takahata Yoshihito2, and Xu Jiabing1   

  1. (1 Beijing Vegetable Research Centre, Beijing 100089, China; 2Faculty of Agriculture, Iwate University , Morioka 020- 8550, Japan)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-08-25 Published:2002-08-25

摘要: 探讨了植物激素、AgNO3 和琼脂浓度对大白菜子叶培养芽再生和植株再生的影响。结果表明:优化培养基条件为MS 培养基中加入5 mg / L BA、0. 5 mg/ L NAA、2 mg/ L AgNO3 和1. 2%~ 1. 6 %琼脂。培养过程中, 子叶的乙烯释放量对芽再生起很重要的作用, 具有高芽再生率的基因型或高浓度琼脂的培养条件下, 子叶产生的乙烯量较少。培养基中有AgNO3 存在时, 尽管子叶的乙烯释放量增加, 芽再生率也有提高。对子叶的乙烯释放量和不定芽再生的关系进行了讨论。

关键词: 大白菜, 子叶培养, AgNO3, 乙烯

Abstract: Effect of hormone combinat ions, AgNO3 and agar concentrat ion on shoot and plant regeneration from cotyledonary explants of Chinese cabbage was described. Maximum shoot regeneration was obtained in MS medium containing 5 mg/ L BA, 0. 5mg/ L NAA, 2 mg/L AgNO3 and solidified with 1. 2 %- 1. 6 % agar. Ethylene production from cultured explants seemed to play important role in shoot regenerat ion. Explants of cultivars with high regenerat ion frequency or if cultured on the medium solidified with higher concentration of agar generally showed low levels of ethylene product ion. However, AgNO3, which also enhanced shoot induction, resulted in an
increase in ethylene production. The possible interaction between ethylene and shoot regenerat ion is discussed.

Key words: Chinese cabbage (Brassica campestris L. ssp. pekinensis), Cotyledon culture, AgNO3, Ethylene

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