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园艺学报 ›› 2002, Vol. 29 ›› Issue (3): 233-237.

• 研究论文 • 上一篇    下一篇

黄瓜花药和花粉高温伤害与多胺和脯氨酸含量的关系

缪 珉;曹碚生   

  1. (扬州大学农学院园艺系, 扬州225009)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-06-25 发布日期:2002-06-25

The Relationship Between Heat Injury and Polyamines or Proline ContentsDuring Anther Development and Pollen Germination in Cucumber

Miao Minmin and Cao Beisheng
  

  1. (Department of Horticulture, Agricultural College of Yangzhou University, Yangzhou 225009, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-06-25 Published:2002-06-25

摘要: 黄瓜花药在开花期比花粉母细胞形成期(阶段1) 和单核花粉期(阶段2) 腐胺、精胺、亚精
胺含量较低, 游离脯氨酸(Pro) 含量较高。高温(35 ℃/ 25 ℃) 使阶段1、阶段2 花药中上述3 种内源多胺含量及开花期花药和花粉中Pro 含量下降。黄瓜花粉在萌发培养基上培养1 h 时3 种内源多胺含量达峰值,此后下降; Pro 含量随着花粉萌发逐步下降; 高温(35 ℃) 下黄瓜花粉萌发后3 种内源多胺含量的上升和Pro 水平的下降趋势被抑制, 但耐热品系XC21、NY21 受高温的影响小于热敏品系新泰密刺或不受影响, 表明多胺和Pro 代谢与耐热性有关。外源鸟氨酸转氨酶(OAT) 的抑制剂3 氨基- 2 , 3 - 二羟基安息香酸(Gabaculine) 能降低黄瓜花粉的萌发能力, 同时提高花粉中内源多胺的含量并降低Pro 的含量, 表明黄瓜花粉萌发过程中可能存在由Pro 合成多胺的途径。

关键词: 黄瓜, 高温, 花药, 花粉, 多胺, 游离脯氨酸

Abstract: Cucumber (Cucumis sativus L. ) anthers had lower Put , Spm, Spd contents and higher free proline (Pro) contents at anthesis than those at pollen mother cell formation stage and monokaryotic pollen stage, high temperature (35 ℃/ 20 ℃) could reduce Put , Spm , Spd contents at stage 1 and stage 2, and reduce Pro contents in anthers and pollens at anthesis. During cucumber pollen culturing, Put, Spm, Spd contents reached max in 1 h then descended, Pro contents descended gradually, Put, Spm, Spd contents decreased and Pro contents increased when cucumber pollen germinated under high temperature ( 35 ℃) . In heat sensitive line Xintaimici , the effects of high temperature on physiological processes above were more serious than those in heat tolerant line XC-1 and NY-1 , which indicated polyamine and Pro might play a key role in cucumber anther development and pollen germination. Gabaculine, a inhibitor of ornithine aminotransferase, could inhibit cucumber pollen germination, improve polyamine contents and reduce Pro contents during pollen germination, which indicated there might be a Pro to polyamine biochemistry route during cucumber pollen germination.

Key words: Cucumber, High temperature, Anther, Pollen, Polyamine, Free proline

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