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园艺学报 ›› 2002, Vol. 29 ›› Issue (2): 168-167.

• 研究报告 • 上一篇    下一篇

梨自交不亲和强度不同品种花柱S糖蛋白含量的差异

张绍铃1;杨记磙2;李秀根2;平伸3;Joseph Ngwela Wolukau1
  

  1. ( 1 南京农业大学园艺学院, 南京210095; 2中国农业科学院郑州果树研究所, 郑州450009; 3 三重大学生物资源学部,日本, 津市514􀀁8507 )
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-04-25 发布日期:2002-04-25

Differences of S-glycoprotein Content in the Styles among Pear Cultivars Differing in Self incompatible Strength

Zhang Shaoling1, Yang Jigun2, Li Xiugen2, Shin Hiratsuka3, and Joseph Ngwela Wolukau1   

  1. ( 1College of Horticulture, Nanjing Agriculture University , Nanj ing 210095, China; 2Zhengz hou Fruit Research I nstitute , CAAS, Zhengzhou 450009, China; 3Faculty of Bioresources , Mie University , Tsu 5148507, Japan)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-04-25 Published:2002-04-25

摘要: 提取梨( Pyrus) 自交不亲和强度不同品种花柱可溶性蛋白质, 应用等电聚焦电泳法在凝胶板
上分离出S 糖蛋白, 用图像解析法进行定量。结果表明, 花柱总S 糖蛋白含量因品种而异, 自交不亲和强、弱及自交亲和的品种每1 􀀁g 花柱可溶性蛋白中含总S 糖蛋白分别为􀀂1. 3 ng 、0. 7 ~ 0. 9 ng、 0. 7 ng 。不同的S 基因所表达的S 糖蛋白量差异显著, 即使是同一S 基因所对应的S 糖蛋白量也因品种而异。

关键词: 梨, 自交不亲和性, S 基因, S 糖蛋白

Abstract: Stylar soluble proteins were extracted from the whole style of pear cultivars differing in self􀀁incom patibility ( SI) strength and were separated by isoelectricfocusing polyacrylamide gel electrphoresis ( IEF􀀁PAGE) and each S-glycoprotein content was quantif ied by an image analyzer. Total S-glycoprotein ( sum of two allelic􀀁linked S glycoproteins) content differed among cultivars. The cult ivars which were strong SI, weak SI and sel􀀁compatibility contained 􀀂1. 3 ng, 0. 7 - 0. 9 ng, and 0. 7 ng of S􀀁glycoprotein per 􀀁g of stylar soluble protein, respectively. S-allele linked glycoprotein content differed greatly in various S-genotypes. Furthermore, the S-glycoprotein content was even different in cultivars with the same S genotype.

Key words: Pear ( Pyrus ), Self􀀁, incompatibility, S allele, S glycoproteins

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