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园艺学报 ›› 2002, Vol. 29 ›› Issue (2): 109-112.

• 研究论文 • 上一篇    下一篇

根癌农杆菌介导绿色荧光蛋白基因转化印度酸桔的研究

石 玮;李东栋;邓秀新3;伊华林   

  1. (华中农业大学作物遗传改良国家重点实验室, 武汉430070)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-04-25 发布日期:2002-04-25

The Transformation of gfp in Cleopatra ( Citrus reticulata ) Mediated byAgrobacterium tumefaciens

Shi Wei , Li Dongdong , Deng Xiuxin , and Yi Hualin   

  1. ( National Key Laboratory in Genetic Improvement of Crops , Huazhong Agriculture University , Wuhan 430070 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-04-25 Published:2002-04-25

摘要: 通过根癌农杆菌介导将绿色荧光蛋白基因转入印度酸桔的胚性愈伤组织中, 经潮霉素筛选,
获得抗性愈伤组织, 并再生植株。对这些植株进行GUS 染色、PCR 分析、绿色荧光检测和Sourthern 杂交验证, 结果表明绿色荧光蛋白已经在转基因植株中表达。

关键词: 柑桔, 愈伤组织, 遗传转化, 绿色荧光蛋白基因, 根癌农杆菌介导

Abstract: The callus of Cleopatra ( Citrus reticulata) , a citrus rootstock cultivar , was transformed by the strain of Agrobacterium tumef aciens EHA105 carrying gfp gene. The callus of Cleopatra ( Citrus reticulata) was co cultivation with A. tumef aciens and selected with hygromycin , as led to the regeneration of resistant embryogenic callus and later to plantlets. The plants were verified GFP2transgenic plants by GUS analysis , GFP2fluorescent detection , PCR amplification and Southern hybridization.

Key words: Citrus, Callus, Green fluorescent protein gene ( gfp), Genetic transformation, Agrobacteriummediated

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