墨兰、春兰变种和品种间同工酶分析

园艺学报 ›› 2002, Vol. 29 ›› Issue (1): 75-77.

墨兰、春兰变种和品种间同工酶分析

孙彩云；张明永；梁承邺；叶秀^*

(中国科学院华南植物研究所, 广州510650)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-02-25 发布日期:2002-02-25

Analysis of Isozyme in Varieties and Cultivars of Cymbidium sinense and Cym bidium goeringii

Sun Caiyun, Zhang Mingyong, Liang Chengye, and Ye Xiulin

(South China I nstitute of Botany, Academia Sinica, Guangzhou 510650)

Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

摘要/Abstract

摘要： 通过酯酶( EST) 、苹果酸酶(ME) 、磷酸葡萄糖异构酶;PGI ( NADP) 、磷酸葡萄糖变位酶
PGM( NAD) 、超氧化物歧化酶( SOD) 5 种酶系统研究墨兰和春兰17 个变种及品种的同工酶多样性, 并探讨这些材料间的亲缘关系。5 种酶系统所得的11 个位点中7 个是多态性位点。EST、PGM( NAD) 和SOD具有多态性。上述5 种酶系统能够把供试品种和变种区分开, 并划分为墨兰和春兰类。

关键词: 墨兰, 春兰, 同工酶, 亲缘关系

Abstract: Five isozyme systems, EST, ME, PGI( NADP) , PGM( NAD) and SOD, were used to study the variation of isozyme in varieties and cult ivars of Cymbidium sinense and C. goeringii as well as the relationships a mong these taxa. Eleven loci were generated. Seven of them were polymorphic. EST, PGM( NAD) , SOD were polymorphic, two of them could be used to distinguish all of varieties and cultivars. The result showed that these isozyme systems could be used to distinguish cult ivars and varieties clearly, and these cultivars and varieties were divided into C. goeringii and C. sinense.

Key words: Cymbidium sinense, Cymbidium goeringii, Isozyme, Variation, Relat ionship

中图分类号:

S 68􀀁

孙彩云;张明永;梁承邺;叶秀. 墨兰、春兰变种和品种间同工酶分析[J]. 园艺学报, 2002, 29(1): 75-77.

Sun Caiyun;Zhang Mingyong;Liang Chengye;and Ye Xiulin. Analysis of Isozyme in Varieties and Cultivars of Cymbidium sinense and Cym bidium goeringii[J]. ACTA HORTICULTURAE SINICA, 2002, 29(1): 75-77.

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