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园艺学报 ›› 2002, Vol. 29 ›› Issue (1): 60-62.

• 研究报告 • 上一篇    下一篇

‘红地球’葡萄叶片、叶柄不定芽再生体系的建立

李 云;冯 慧*;田砚亭

  

  1. (北京林业大学生物科学与技术学院, 北京100083)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-02-25 发布日期:2002-02-25

Study on Red Globe Grape Leaf and Petiole Adventitious Bud RegeneratingSystem

Li Yun, Feng Hui, and Tian Yanting
  

  1. (College of Biology and Biotechnology, Beijing Forestry University, Beij ing 100083)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

摘要: 探讨了多种因素对红地球葡萄试管苗叶片、叶柄不定芽再生的影响, 叶片再生与生长调节物质种类和浓度、叶片着生部位等因子有关, 叶片不定芽再生的最佳培养基为NN69+ BA 2. 0 ~ 2. 5 mg/L+ IBA 0. 2 mg/L, 不定芽再生率以顶端第1 片幼叶最高。

关键词: 葡萄, 再生系统, 不定芽

Abstract: It is necessary to establish an efficient regenerating system before genetic transformation of grapevine. In this study, an efficient regenerat ing system was established. Advent itious buds were obtained from the leaves of Red Globe ( Vitis vinif era L. ) , and the factors that affect adventit ious bud regeneration were studied. The regenerating rate of adventitious bud had relationship with the kinds and the concentrat ion of plant growth regu lator, leaf position, etc. The opt imum medium for advent itious bud regeneration was found, which is NN69+ BA 2. 0- 2. 5 mg/ L+ IBA 0. 2 mg/ L. The regenerating rate of the adventitious bud from the first top leaf was the
highest.

Key words: Grape, Regenerat ing system, Adventit ious bud

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