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园艺学报 ›› 2002, Vol. 29 ›› Issue (1): 13-19.

• 果树 • 上一篇    下一篇

板栗主栽品种的遗传多样性及其亲缘关系分析

暴朝霞;黄宏文*
  

  1. ( 中国科学院武汉植物研究所, 武汉430074)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-02-25 发布日期:2002-02-25

Analysis of Genetic Diversity and Genetic Relationships of Chinese Chestnut

Bao Zhaoxia andHuang Hongwen   

  1. (Wuhan Institute of Botany , the Chinese Academy of Sciences , Wuhan 430074)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

摘要: 采用9 个酶系统的15 个同工酶位点, 对89 个板栗品种进行了遗传多样性分析, 并以品种间
的遗传距离构建UPGMA 聚类图, 鉴别板栗品种和评价它们之间的遗传关系。结果表明: ( 1) 我国板栗主要产区遗传多样性较高, 如浙江、山东、湖北和江苏省; ( 2) 在供试的89 个板栗品种中, 除5 个品种外, 其它均可用多位点同工酶对其作专一性鉴定; ( 3) 基于品种间等位酶遗传距离的UPGMA 聚类图将山东、湖北、江苏以及河南的大部分板栗品种分别聚在一起, 体现了在遗传构成上同地域的板栗品种具有遗传关系相近的特征。

关键词: 板栗, 同工酶, 遗传多样性, 品种鉴定, 聚类分析

Abstract: Eighty-nine tradit ional cultivars of Chinese chestnut were investigated in present study for genet icdiversity, cultivar identification and genet icrelationships. Fifteen isozyme loci of 9 enzymes were examined using isoelectric focusing in thin􀀁layer polyacrylamide gels. The parameters of genetic diversity and genetic distance were calculated by Biosys 1 and henogram was constructed by UPGMA clustering analysis with NTSYS software. A high genetic diversity was observed in major chestnut production provinces, such as Zhejiang, Shandong, Hubei and Jiangsu. Eighty􀀁four out of total 89 cultivars examined can be uniquely identified by 15 multi􀀁locus isozymes. The
UPGMA phenogram showed that the majority of cultivar of Shandong, Hubei, Jiangsu and Henan province was clustered together. The traditional cult ivars of Chinese chestnut were inclined to clustering of provincial groups.

Key words: Abstract: Key words: Chinese chestnut, Isozyme, Genetic diversity, Cultivar identification, Cluster analysis

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