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园艺学报 ›› 2002, Vol. 29 ›› Issue (1): 1-4.

• 果树 •    下一篇

一个与苹果属显性矮生主基因Dw连锁的RAPD标记

毕晓颖1;吴禄平1;安利佳2
  

  1. (1 沈阳农业大学园艺系, 沈阳110161 ; 2大连理工大学生化工程研究所, 大连116012)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-02-25 发布日期:2002-02-25

A RAPD Marker Linked to a Dominant Dwarf Gene in Apple

Bi Xiaoying1 , Wu Luping1 , and An Lijia2
  

  1. (1 Shenyang Agriculture University , Shenyang 110161; 2 Dalian University of Technology , Dalian 116012)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

摘要: 运用RAPD 技术, 采用集群分析法进行了苹果抗寒矮化种质资源扎矮76 〔Malus baccata (L. )Borkh. 〕显性矮生主基因Dw连锁的分子标记研究。研究结果表明, 扎矮76 的矮生性状由显性单基因控制, RAPD 标记OPE1521001 与Dw基因连锁, 其连锁距离为0. 69 cM。这为最终定位及克隆该矮生基因打下基础。

关键词: 苹果属, Dw基因, RAPD 标记, 矮生

Abstract: Randomly Aplified Polymorphic DNA (RAPD) was employed to detect a molecular marker linked to the dominant dwarf gene in apple. A RAPD marker OPE1521001was proved to be linked to the dwarf gene. The genetic distance is 0. 69 cM. This work has provided a basis for locating and cloning Dw gene in the future.

Key words: Apple, Dwarf gene, RAPD marker

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