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园艺学报 ›› 2011, Vol. 38 ›› Issue (1): 43-54.

• 果树 • 上一篇    下一篇

杏EST-SSR标记的开发

上官凌飞1,李晓颖1,宁 宁1,2,王玉柱2,*,章 镇1,房经贵1   

  1. (1南京农业大学园艺学院,南京 210095;2北京市农林科学院林业果树研究所,北京 100093)
  • 收稿日期:2010-04-01 修回日期:2010-11-05 出版日期:2011-01-25 发布日期:2011-01-25
  • 通讯作者: 王玉柱

Development of EST-SSR Markers in Apricot

SHANGGUAN Ling-fei1,LI Xiao-ying1,NING Ning1,2,WANG Yu-zhu2,*,ZHANG Zhen1,and FANG Jing-gui1   

  1. (1College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2Institute of Forestry Pomology Academy of Agricultural and Forestry Sciences,Beijing 100093,China)
  • Received:2010-04-01 Revised:2010-11-05 Online:2011-01-25 Published:2011-01-25
  • Contact: WANG Yu-zhu

摘要: 利用MISA软件对NCBI上杏的15 387条EST序列进行SSR位点查找,发掘出含有SSR位点的序列1 073条,共有1 353个SSR位点,候选SSR位点出现的频率为8.79%。二核苷酸、三核苷酸和四核苷酸重复是最主要的重复类型,分别占23.50%、38.80%和23.43%。设计了50对EST-SSR引物并进行PCR扩增,发现40对引物能扩增出理想的PCR 产物,其中有25对引物能够扩增出多态性带。随机对7对引物的部分杏扩增片段进行了测序分析,发现有57.1%的片段具有相应的SSR位点,对梅DNA指纹中部分谱带的测序结果也证明是杏扩增出的相应的SSR位点。根据推算,从杏EST中开发SSR标记的开发效率为3.98%。进一步选用20对扩增效果最好的引物对24个杏品种,16个花梅品种以及8个果梅品种进行DNA指纹构建与遗传多样性分析,结果表明,来源于杏的EST-SSR引物在梅中有很高的通用性,杏和梅是遗传差异明显的两种植物,果梅与花梅在遗传上是不能分为两类的同种植物。

关键词: 杏, 梅, EST-SSR, 遗传差异分析

Abstract: 15 387 ESTs of apricot from NCBI were screened using MISA software. The results showed that 1 353 SSRs were mined from 1 073 ESTs with a frequency of 8.79%. Di-,tri- and tetra nucleotide repeats EST-SSRs were dominant,accounting for 23.50%,38.80% and 23.43%,respectively. Fifty primer pairs were designed for partial EST-SSRs and used for PCR amplification with 40 primer pairs showing amplifications while 25 were polymorphic. PCR products from 7 EST-SSR primer pairs were selected for sequence analysis where 57.1% of the fragments contained SSRs. EST-SSR motifs were conserved between mei and apricot. Accordingly,the proportion of developing efficient EST-SSR primers from apricot was 3.98%. The best twenty workable EST-SSR primer pairs were chosen to generate DNA fingerprints of 24 apricot,16 flower-mei and 8 fruit-mei cultivars. Using these EST-SSR primer pairs,mei and apricot were classified into 2 groups. The EST-SSR primers designed from apricot sequences showed high polymorphism in mei varieties and exhibited high transferability between mei and apricot cultivars;Fruit-mei and flower-mei were genetically the same plant.

Key words: apricot, mei, EST-SSR, analysis of genetic diversity

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