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园艺学报 ›› 2010, Vol. 37 ›› Issue (9): 1499-1506.

• 研究报告 • 上一篇    下一篇

中国板栗种质资源的AFLP分析

兰彦平,周连第*,姚研武,王尚德,刘国彬   

  1. (北京市农林科学院农业综合发展研究所,北京 100097)
  • 收稿日期:2010-03-09 修回日期:2010-06-25 出版日期:2010-09-25 发布日期:2010-09-25
  • 通讯作者: 周连第

Analysis of Castanea mollissima Germplasm Resources by AFLP

LAN Yan-ping,ZHOU Lian-di*,YAO Yan-wu,WANG Shang-de,and LIU Guo-bin   

  1. (Institute of Agricultural Integrated Development,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
  • Received:2010-03-09 Revised:2010-06-25 Online:2010-09-25 Published:2010-09-25
  • Contact: ZHOU Lian-di

摘要: 采用荧光标记AFLP技术,利用筛选出的9对EcoRⅠ/MseⅠ引物对90份中国板栗种质进行遗传变异及遗传关系分析。90份种质共产生806条带,695条多态性条带,多态比率86.23%;基于种质间遗传相似性系数的UPGMA聚类图将90份种质聚为两大类14个小组;9对引物在90个板栗种质中检测到数量不等的品种特异带型,各引物对对供试材料具有较高的鉴别效率,最高93%,最低80%。引物对两两组合可完全区分所有供试材料。

关键词: 板栗, 亲缘关系, AFLP, 种质鉴定

Abstract: The genetic variation and genetic relationships of 90 Castanea mollissima cultivars were analyzed by the fluorescent-AFLP technique with 9 primer combinations. Among the 806 bands detected,695 bands were polymorphic,and the high percentage of polymorphic loci(86.23%)revealed a high genetic diversity among the 90 C. mollissima cultivars. The UPGMA phenogram based on genetic identity clustered the 90 C. mollissima cultivars into 2 groups and 14 rows. The 9 pairs of AFLP primer combination used could distinguish all the tested cultivars,the highest ratio of identification is 93% and the lowest is 80%.

Key words: Castanea mollissima, genetic relationship, AFLP, germplasm identification

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