关键词: 马铃薯, 离体茎尖, 小滴玻璃化法, 超低温保存, 遗传稳定性

Abstract: Shoot tips from axillary buds of in vitro potato（Solanum tuberosum L.）were successfully cryopreserved by droplet vitrification. The optimum cryopreservation procedures were as follows. Shoot tips excised from 2–3 month-old plantlets were precultured on liquid MS medium supplemented with 0.3 mol · L-1 and 0.5 mol · L-1 sucrose for 1 day each and then dehydrated with PVS2 for 30 min at 0 ℃. Five shoot tips were transferred to approximately 15 µL droplets of PVS2 solution on thin strips of sterile aluminum foil. The aluminum foil strips were folded to enclose the shoot tips. The foil envelope was then carefully immersed into liquid nitrogen（LN）using fine forceps. After immersion the strips were quickly transferred to 2 mL cryotubes and immediately plunged into LN and maintained for 1 h at least. The shoottips in the foil strips were then rapidly heated by immersion twice at 15 min intervals into 10 mL 1.2 mol · L-1 sucrose MS medium at room temperature. The shoot tips were then unloaded from the foil，rinsing the PVS2 from the shoot tips. The shoot tips were transferred to solid culture medium（MS + 0.5 mg · L-1 Zeatin + 0.1 mg · L-1 NAA + 1.0 mg · L-1 GA3）for recovery and regeneration. The average survival rate and regeneration rate were 79.91% and 62.52%，respectively. There was no genetic variation in the regenerated plants based on assessment of SSR markers.

Key words: potato, In vitro shoot tips, droplet vitrification method, cryopreservation, genetic stability