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园艺学报 ›› 2010, Vol. 37 ›› Issue (9): 1416-1422.

• 蔬菜 • 上一篇    下一篇

分子标记辅助聚合番茄抗病基因育种

朱明涛1,孙亚林1,郑 莎1,张晓黎1,王涛涛1,叶志彪2,李汉霞1,*   

  1. (1华中农业大学园艺植物生物学教育部重点实验室,武汉430070;2作物遗传改良国家重点实验室,武汉430070)
  • 收稿日期:2010-05-25 修回日期:2010-08-02 出版日期:2010-09-25 发布日期:2010-09-25
  • 通讯作者: 李汉霞

Pyramiding Disease Resistance Genes by Molecular Marker- Assisted Selection in Tomato

ZHU Ming-tao1,SUN Ya-lin1,ZHENG Sha1,ZHANG Xiao-li1,WANG Tao-tao1,YE Zhi-biao2,and LI Han-xia1,*   

  1. (1College of Horticulture & Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;2National Key Laboratory of Crop Genetic Improvement,Wuhan 430070,China)
  • Received:2010-05-25 Revised:2010-08-02 Online:2010-09-25 Published:2010-09-25
  • Contact: LI Han-xia

摘要: 利用番茄抗病基因Tm-2a、I-2、Mi-1、Cf-9的分子标记辅助选择,聚合抗烟草花叶病、抗枯萎病、抗根结线虫和抗叶霉病4个基因于番茄育种材料中。室内苗期接种鉴定和田间观察,获得的含4个抗性基因的株系L11、L19、L46和L51对4种病害都达到了抗级(R)水平,且具有较好的农艺性状,可作为抗病育种亲本。

关键词: 番茄, 分子标记, 抗病性, 聚合育种

Abstract: The elite lines of L11,L19,L46 and L51 containing Tm-2a,I-2,Mi-1 and Cf-9 resistant genes were obtained in tomato with the aid of the molecular marker assisted selection. Seedling inoculation of these inbred lines confirmed higher disease resistant levels. The results of field trials also showed that the four selected disease resistance lines have better agricultural characteristics and may be used for tomato disease resistance breeding program in the future.

Key words: tomato, marker-assisted selection, disease resistance, pyramiding breeding

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