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园艺学报 ›› 2010, Vol. 37 ›› Issue (8): 1227-1234.

• 果树 • 上一篇    下一篇

葡萄离体叶片和果实白藜芦醇及其糖苷合成对UV-C辐射诱导的响应

郑先波1,2,李晓东2,吴本宏2,王利军2,刘春艳2,李绍华3,*   

  1. (1河南农业大学园艺学院,郑州 450002;2中国科学院植物研究所,北京 100093;3中国科学院武汉植物园,武汉 430074)
  • 收稿日期:2010-03-03 修回日期:2010-05-14 出版日期:2010-08-25 发布日期:2010-08-25
  • 通讯作者: 李绍华

Synthesis of Resveratrol and Piceid in Different Grape Organs in Response to UV-C Irradiation

ZHENG Xian-bo1,2,LI Xiao-dong2,WU Ben-hong2,WANG Li-jun2,LIU Chun-yan2,and LI Shao-hua3,*   

  1. (1Horticulture College,Henan Agricultural University,Zhengzhou 450002,China;2Institute of Botany,the Chinese Academy of Sciences,Beijing 100093,China;3Wuhan Botanical Garden,the Chinese Academy of Sciences,Wuhan 430074,China)
  • Received:2010-03-03 Revised:2010-05-14 Online:2010-08-25 Published:2010-08-25
  • Contact: LI Shao-hua

摘要: 以‘北丰’和‘北全’葡萄离体叶片和果实为试材,研究了葡萄不同器官内白藜芦醇(Res)及其糖苷合成对紫外线C(UV-C)辐射诱导的响应,结果表明:不同器官反式白藜芦醇(trans-Res)合成积累对UV-C辐射诱导反应存在差异。叶片适宜的UV-C辐射剂量为1.8 kJ · m-2,而果皮为1.8 ~ 5.4 kJ · m-2,果实Res合成积累对UV-C辐射诱导的敏感性低于叶片。3.6 kJ · m-2 UV-C辐射果粒对种子中trans-Res含量没有显著影响,直接辐射完整种子或剖开种子切面也不能显著提高trans-Res含量。UV-C辐射处理的叶片和果皮中trans-Res含量随辐射后常温避光孵育时间的延迟表现出先升后降的趋势,而反式白藜芦醇苷(trans-PD)和顺式白藜芦醇苷(cis-PD)含量在12 ~ 48 h内迅速提高,之后维持在相对稳定状态。种子中trans-Res含量在整个孵育过程中与对照之间均不存在显著差异。

关键词: 葡萄, 白藜芦醇, 白藜芦醇苷, 紫外线辐射

Abstract: Resveratrol(Res)and piceid(PD)synthesis in leaves,berry skin,and seeds in‘Beifeng’and‘Beiquan’grapes in response to UV-C irradiation were studied. In addition,the dynamic changes of Res and PD content in berry skin and seeds with the extension of storage time at room temperature under dark conditions were investigated. The results showed trans-resveratrol(trans-Res)synthesis in various organs in response to UV-C irradiation was different. The optimal dosage of UV-C irradiation for leaves was 1.8 kJ · m-2,while for berry skin it ranged from 1.8 to 5.4 kJ · m-2,and Res synthesis in leaves was more sensitive than that in fruits in response to UV-C irradiation. Trans-Res content in grape seeds was not significantly changed when grape berries,excised seeds,and dissected seeds were directly irradiated with 3.6 kJ · m-2 UV-C. Trans-Res content in UV-C treated grape berry skin and leaves increased rapidly andthen decreased sharply with the extension of storage time at room temperature in the dark. Trans-PD and cis-PD content in UV-C treated grape berry skin and leaves increased rapidly during the first 12–48 h storage,and then maintained a relatively stable level. There was no difference in trans-Res content between the UV-C treated grape seeds and the control grape seeds.

Key words: grape, resveratrol, piceid, UV-C irradiation

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