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园艺学报 ›› 2005, Vol. 32 ›› Issue (4): 643-647.

• 研究论文 • 上一篇    下一篇

西瓜杂交种遗传多态性的SRAP标记分析

李严;张春庆   

  1. (山东农业大学农学院, 泰安271018)
  • 收稿日期:2004-11-11 修回日期:2005-01-12 出版日期:2005-08-25 发布日期:2005-08-25

Studies on Genetic Diversity with a Molecular Marker SRAP of WatermelonHybrids

Li Yan;Zhang Chunqing   

  1. (College of Agronomy, Shandong Agricultural University, Tai'an 271018, China)
  • Received:2004-11-11 Revised:2005-01-12 Online:2005-08-25 Published:2005-08-25

摘要: 利用新型分子标记SRAP ( Sequence2Related Amp lified Polymorphism) 进行西瓜杂交种遗传多态性的研究, 利用25个引物组合对当前生产上推广的20个西瓜杂交种进行扩增, 从中筛选得到20个多态性引物组合, 共产生135个多态性条带, 平均每个引物组合产生71.1个多态性条带, 显示了较高的多态性比率。聚类分析20个引物组合的扩增结果, 20份材料分为3大类, Jaccardps相似系数在0.29~0.86之间。

关键词: 西瓜, 杂交种, 多态性, SRAP

Abstract: Study on genetic diversity of watermelon could benefit the research of cultivar property right p rotecting, seed purity testing, and genetic breeding. A new molecularmarker system-SRAP ( Sequence-Related Amplified Polymorphism) was firstly app lied on 20 watermelon hybrids genetic diversity research. 20
primer pairs selected from 25 amplified 135 polymorphic bandswith an average of 71.1 polymorphic bands per primer pair. Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 20 primer pairs showed 20 hybrids could be distinguished into three main groups. Jaccard's similarity coefficient ranged from 0.29 - 0.86.

Key words: Watermelon, Hybrid, Polymorphism, SRAP

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