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园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1509-1514.

• 观赏植物 • 上一篇    下一篇

三倍体狗牙根染色体数变异的分子细胞学鉴定

龚志云;高清松;苏 艳;单丽丽;于恒秀;王 淼;裔传灯;顾铭洪*
  

  1. (扬州大学, 江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室,江苏扬州225009)
  • 收稿日期:2007-07-09 修回日期:2007-09-30 出版日期:2007-12-25 发布日期:2007-12-25
  • 通讯作者: 顾铭洪

Molecular-cytological Identification of Triploid Bermudagrass Chromosome Number Variation

GONG Zhi-yun, GAO Qing-song, SU Yan, SHAN Li-li, YU Heng-xiu, WANG Miao, YI Chuan-deng, and GU Ming-hong*
  

  1. (Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/ Key Laboratory of Plant Functional Genomics, Ministry of Education,Yangzhou University, Yangzhou, Jiangsu 225009, China)
  • Received:2007-07-09 Revised:2007-09-30 Online:2007-12-25 Published:2007-12-25

摘要: 对三倍体狗牙根‘矮生百慕大’进行细胞学研究,结果表明,其细胞内染色体数存在非整倍性变异,变化范围为2n=27~30。进一步利用荧光原位杂交(FISH)技术以45S rDNA为探针进行鉴定,发现在三倍体狗牙根‘矮生百慕大’细胞中有3条染色体含有45S rDNA,并且都位于着丝粒部位。这3条染色体上的杂交信号强弱不一,两个染色体在45S rDNA区杂交信号较强,且易伸长呈线状结构,杂交信号较弱的一条染色体可能由于45S rDNA含量比较少,所以这一区域染色体很易在分裂中"拉长"并断裂,形成非整倍体。

关键词: 狗牙根, 多倍体, 45S rDNA, 荧光原位杂交

Abstract: Cytological investigation of the triploid bermudagrass showed chromosome number varies between 27 and 30 in somatic cell indicating that some of the plants were aneuploid. In order to study the chromosome variation, the digoxigenin-11-dUTP labeled 45S rDNA was used. Three distictive signals were locating to the triploid bermudagrass prometaphase chromosome centromeres by fluorescence in situ hybridization (FISH). The intensity of three signals was different, and two signals of them showed lines sometimes. The chromosome with the weakest signal was easy to snap, and aneuploid appeared.

Key words: Bermudagrass, Polyploid, 45S rDNA, Fluorescence in situ hybridization