关键词: 梨, 花柱, 花粉, RNA, 降解, 自交不亲和性

Abstract: The RNAs were isolated from styles that were self- and cross-pollinated with ³² P-labelled pollens in vivo. Than radioactivity of pollinated style RNA was determined with a liquid scintillation counter, and agarose gel electrophoresis was used to test the degradation of RNA from pollinated styles. Results were
that the content of pollen ³² P-RNA in self-pollinated style RNA is 40.7% - 25.3% that of pollen ³² P-RNA in cross-pollinated style RNA after pollination 12 - 48 h, and that pollen intact RNA in self-pollinated style was specifically decreased. This indicated that pollen RNA in self-pollinated style was specifically degraded.

Key words: Pear, Style, Pollen, RNA, Degradation, Self2incompatibility