https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2005, Vol. 32 ›› Issue (5): 885-888.

• 研究报告 • 上一篇    下一篇

番茄酸性转化酶cDNA片段的克隆与表达分析

姜 晶;李天来;李 伟   

  1. (沈阳农业大学园艺学院, 辽宁省设施园艺重点实验室, 沈阳110161)
  • 收稿日期:2004-12-22 修回日期:2005-04-11 出版日期:2005-10-25 发布日期:2005-10-25

Cloning and Expression Analysis of Acid Invertase Gene cDNA Fragment from Tomato

Jiang Jing, Li Tianlai;Li Wei   

  1. (College of Horticulture, Shenyang Agricultural University, Liaoning Key Laboratory of Protected Horticulture, Shenyang 110161,China)
  • Received:2004-12-22 Revised:2005-04-11 Online:2005-10-25 Published:2005-10-25

摘要: 根据番茄细胞壁束缚性和可溶性酸性转化酶基因序列分别设计引物, 以普通栽培型番茄(Lycopersicon esculentum ) ‘辽园多丽’和‘桃太郎’及野生番茄(Lycopersicon chmielewskii) 的幼苗为材料提取总RNA, 采用RT2PCR方法, 扩增出目的cDNA序列的部分片段并克隆到pMD18-T载体中。经测序后发现不同基因型番茄的酸性转化酶基因具有高度同源性, 达到99.5%。进一步检测其在‘辽园多丽’果实发育不同时期的表达变化, 结果表明主要在成熟果实的胶质胎座中大量表达, 而在果实的其它部位几乎检测不到其表达。

关键词: 番茄, 酸性转化酶, RT-PCR, 序列分析, 基因表达

Abstract: According to the published gene sequences of cell wall2binding and soluble acid invertase of tomato, two pairs of primers were designed respectively. The cDNA fragments were obtained by reverse transcriptional polymersae chain reaction (RT-PCR) using the whole RNA of different genotype tomatos, ‘Liaoyuan Duoli’, ‘Momotaro’and wild type (Lycopersicon chmielewskii) , as the templates. The fragments were cloned into pMD18-T vector and then sequenced. The sequences showed highly homology ( to 99.5% )to the published sequences of soluble and insoluble invertases, respectively. Northern blotting was performed to analyze the exp ression pattern of acid invertase in different development stages of tomato fruit of‘Liaoyuan Duoli’. The result showed that soluble acid invertase was highly expressed in pectinic placenta of mature tomato fruit, but no expression was detected in other parts of fruits.

Key words: Tomato, Acid invertase, RT-PCR, Sequence analysis, Gene expression

中图分类号: