https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
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园艺学报 ›› 2005, Vol. 32 ›› Issue (6): 1102-1104.

• 研究报告 • 上一篇    下一篇

黄瓜枯萎病新型抑制蛋白L37的研究

郑爱萍;闫 敏;李 平;谭芙蓉;郑秀丽;李 壮   

  1. (四川省农业生物技术工程研究中心, 成都611130)
  • 收稿日期:2005-03-21 修回日期:2005-05-16 出版日期:2005-12-25 发布日期:2005-12-25

Research on New Antagonistic Prote in L37 against Fusarium oxysporum

Zheng Aiping;Yan Min;Li Ping;Tan Furong;Zheng Xiuli;Li Zhuang   

  1. (Sichuan Provincial Center for Agri-Biotech Research, Chengdu 611130, China)
  • Received:2005-03-21 Revised:2005-05-16 Online:2005-12-25 Published:2005-12-25

摘要: 从土壤中分离到对黄瓜枯萎病病原菌有抑制作用的枯草芽孢杆菌(Bacillus subtilis) , 发酵产物经DEAE - 纤维素、Sephadex G-100柱层析, 分离到15 kD抑菌蛋白L37, SDS-PAGE显示纯度达到电泳纯, N - 末端氨基酸序列同源性检测结果提示可能是一种新型的抑菌蛋白。蛋白质抑菌结果表明, 对多种病原真菌具有抑制效果, 且对蛋白酶、温度和大部分有机物不敏感, 是稳定的抑菌蛋白。对病原菌菌丝抑制机理研究表明, 抑菌蛋白具有严重扭曲、断裂、阻止生长等抗生效果。应用发酵蛋白产物进行两年大田防效试验表明, 对黄瓜枯萎病防效显著, 最高达到90% , 具有显著提高产量的作用。

关键词: 黄瓜, 黄瓜枯萎病, 枯草芽孢杆菌, 抑菌蛋白, 防治

Abstract: Bacillus subtilis inhibiting cucumberwither pathogen was screened from soil. After fermenting, 15 kD antagonistic protein named L37 was got through DEAE-fibrin, Sephadex G-100 chomatography, which proved purified completely through SDS-PAGE. It was probably a new type of antagonistic p rotein against
pathogen by the sequence on the amino N-terminal testing, which could make the normal hypha of pathogen distort, split and inhibit. The control effect could reach 90% against cucumberwither pathogen and improved the yield in field controlling test by fermenting products.

Key words: Cucumber, Cucumber wither, Bacillus subtilis, Antagonistic protein, Controlling

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